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Mouse G-CSF, C-His Tag Protein

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Usd: 379

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Catalog# HA210972

Mouse G-CSF, C-His Tag Protein

Product Information

Product name

Mouse G-CSF, C-His Tag Protein

Catalog No.

HA210972

Predicted molecular mass

20.3 kD

Protein construction description

A DNA sequence encoding the mouse G-CSF protein (P09920) (Val 31-Ala 208) was expressed with a His tag at the C-terminus.

Accession

P09920

Protein construction

G-CSF (31-208) His

Source

HEK293

Bio Activity

Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 10-60 pg/mL.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Mouse

Background

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.