IL-12RB2 Rabbit Polyclonal Antibody
Catalog# HA500320
IL-12RB2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
IL-12RB2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IL-12RB2 aa 751-800.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 97 kDa
Positive Control
HepG2 cell lysate, 293T cell lysate, Hela, human placenta tissue, THP-1.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:200
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IHC-P
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1:600
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FC
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1:500-1:1,000
Target
Function
Interleukin 12 receptor, beta 2 subunit is a subunit of the interleukin 12 receptor. IL12RB2 is its human gene. IL12RB2 orthologs have been identified in all mammals for which complete genome data are available. The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The co-expression of this and IL12Rβ1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. While the IL12Rβ1 subunit is constitutively expressed, the expression of the IL12RB2 gene is up-regulated by interferon gamma. In Th1 cells, IL-12 signaling through the IL12 receptor leads to the phosphorylation of STAT4 and continued Th1 differentiation. The IL12Rβ2 subunit plays an important role in Th1 cell differentiation, since its absence leads to an abortive Th1 differentiation that has dysfunctional production of Th1 effector molecules. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense.
Background References
1. Prigione I. et. al. IL12RB2 Polymorphisms correlate with risk of lung adenocarcinoma. Immunobiology. 2016 Feb
2. Kato-Kogoe N. et. al. Functional analysis of differences in transcriptional activity conferred by genetic variants in the 5\' flanking region of the IL12RB2 gene. Immunogenetics. 2016 Jan
Subcellular Location
Membrane.
UNIPROT
Synonyms
I12R2_HUMAN antibody
IL-12 receptor subunit beta-2 antibody
IL-12R subunit beta-2 antibody
IL-12R-beta-2 antibody
IL-12RB2 antibody
IL12 receptor beta 2 antibody
IL12R beta2 antibody
IL12RB2 antibody
Interleukin 12 receptor beta 2 antibody
Interleukin 12 receptor beta 2 chain antibody
ExpandImages
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Western blot analysis of IL-12RB2 on different lysates with Rabbit anti-IL-12RB2 antibody (HA500320) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500320) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling IL-12RB2 with Rabbit anti-IL-12RB2 antibody (HA500320) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-12RB2 antibody (HA500320) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-IL-12RB2 antibody (HA500320) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500320) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of THP-1 cells labeling IL-12RB2.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500320, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"