SCD1 Recombinant Mouse Monoclonal Antibody [A9A11-R] - BSA and Azide free
Catalog# HA610063
SCD1 Recombinant Mouse Monoclonal Antibody [A9A11-R] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SCD1 Recombinant Mouse Monoclonal Antibody [A9A11-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human SCD1 aa 310-509 / 509.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, A431 cell lysate, A549 cell lysate, mouse liver tissue lysate, rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
A9A11-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Stearoyl-CoA desaturase (Δ-9-desaturase) is an endoplasmic reticulum enzyme that catalyzes the rate-limiting step in the formation of monounsaturated fatty acids (MUFAs), specifically oleate and palmitoleate from stearoyl-CoA and palmitoyl-CoA. Oleate and palmitoleate are major components of membrane phospholipids, cholesterol esters and alkyl-diacylglycerol. In humans, the enzyme is encoded by the SCD gene. Stearoyl-CoA desaturase-1 is a key enzyme in fatty acid metabolism. It is responsible for forming a double bond in Stearoyl-CoA. This is how the monounsaturated fatty acid oleic acid is produced from the saturated fatty acid stearic-acid. A series of redox reactions, during which two electrons flow from NADH to flavoprotein cytochrome b5, then to the electron acceptor cytochrome b5 as well as molecular oxygen introduces a single double bond within a row of methylene fatty acyl-CoA substrates. The complexed enzyme adds a single double bond between the C9 and C10 of long-chain acyl-CoAs from de-novo synthesis. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with oxidation of a pair of donors resulting in the reduction of O to two molecules of water. The systematic name of this enzyme class is stearoyl-CoA,ferrocytochrome-b5:oxygen oxidoreductase (9,10-dehydrogenating). Other names in common use include Delta9-desaturase, acyl-CoA desaturase, fatty acid desaturase, and stearoyl-CoA, hydrogen-donor:oxygen oxidoreductase. This enzyme participates in polyunsaturated fatty acid biosynthesis and ppar signaling pathway. It employs one cofactor, iron.
Background References
1. Ye Z et al. FBW7-NRA41-SCD1 axis synchronously regulates apoptosis and ferroptosis in pancreatic cancer cells. Redox Biol. 2021 Jan
2. Xuan Y et al. SCD1/FADS2 fatty acid desaturases equipoise lipid metabolic activity and redox-driven ferroptosis in ascites-derived ovarian cancer cells. Theranostics. 2022 Apr
Subcellular Location
Endoplasmic reticulum membrane.
Synonyms
ACOD_HUMAN antibody
Acyl-CoA desaturase antibody
Delta(9)-desaturase antibody
Delta-9 desaturase antibody
Delta-9-Desaturase antibody
FADS5 antibody
Fatty acid desaturase antibody
PRO0998 antibody
SCD 1 antibody
SCD antibody
ExpandImages
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This data was developed using HA601180, the same antibody clone in a different buffer formulation.
Western blot analysis of SCD1 on different lysates with Mouse anti-SCD1 antibody (HA601180) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: SK-MEL-28 cell lysate
Lane 5: A375 cell lysate
Lane 6: A431 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 37 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601180) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA601180, the same antibody clone in a different buffer formulation.
Western blot analysis of SCD1 on different lysates with Mouse anti-SCD1 antibody (HA601180) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 37 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601180) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
SCD1 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
SCD1 Rabbit Polyclonal Antibody
Application: IHC-P,IF-Cell,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
