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Transmembrane protein 200A Recombinant Mouse Monoclonal Antibody [4-B3-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610071

Transmembrane protein 200A Recombinant Mouse Monoclonal Antibody [4-B3-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Transmembrane protein 200A Recombinant Mouse Monoclonal Antibody [4-B3-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human Transmembrane protein 200A aa 59-359 / 491.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 54 kDa

Positive Control

A549 cell lysate, Caco-2 cell lysate, PC-3M cell lysate.

Conjugation

unconjugated

Clone Number

4-B3-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Transmembrane protein 200A is a protein that in humans is encoded by the TMEM200A gene.

Background References

1. Hui A B Y et al. Identification of a novel homozygous deletion region at 6q23.1 in medulloblastomas using high-resolution array comparative genomic hybridization analysis. Clin Cancer Res 11:4707-4716 (2005).

2. Carninci P et al. The transcriptional landscape of the mammalian genome. Science 309:1559-1563 (2005).

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q86VY9 Human

Synonyms

KIAA1913 antibody

T200A_HUMAN antibody

Tmem200a antibody

Transmembrane protein 200A antibody

TTMC antibody

Two transmembrane C antibody

Images

  • Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody (<a href="/products/HA610071" style="font-weight: bold;text-decoration: underline;">HA610071</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: Caco-2 cell lysate<br />Lane 3: PC-3M cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 45 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610071" style="font-weight: bold;text-decoration: underline;">HA610071</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody (HA610071) at 1/1,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: Caco-2 cell lysate
    Lane 3: PC-3M cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 47 kDa

    Exposure time: 45 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610071) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"