T7 tag Recombinant Mouse Monoclonal Antibody [4-C2-R] - BSA and Azide free
Catalog# HA610073
T7 tag Recombinant Mouse Monoclonal Antibody [4-C2-R] - BSA and Azide free
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WB
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Species independent
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HA601193
含抗保成分
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unconjugated
Overview
Product Name
T7 tag Recombinant Mouse Monoclonal Antibody [4-C2-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide CMASMTGGQQMG.
Species Reactivity
Species independent
Validated Applications
WB
Conjugation
unconjugated
Clone Number
4-C2-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.
Background References
1. Horn AV et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013)
2. Kubota T., et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem. 283:25660-70 (2008).
Synonyms
10 antibody
Major capsid protein 10A antibody
Major capsid protein antibody
Minor capsid protein 10B antibody
T7 epitope tag antibody
Images
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Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (HA610073) at 1/1,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"