NQO1 Recombinant Mouse Monoclonal Antibody [13A1-R] - BSA and Azide free
Catalog# HA610087
NQO1 Recombinant Mouse Monoclonal Antibody [13A1-R] - BSA and Azide free
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WB
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Human
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HA601209
含抗保成分
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unconjugated
Overview
Product Name
NQO1 Recombinant Mouse Monoclonal Antibody [13A1-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human NQO1 aa 1-274 / 274.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 31 kDa
Positive Control
A549 cell lysate, SiHa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HeLa cell lysate.
Conjugation
unconjugated
Clone Number
13A1-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
Background References
1. Jaiswal A K et al. Human NAD(P)H:quinone oxidoreductase (NQO1) gene structure and induction by dioxin. Biochemistry 30:10647-10653 (1991).
2. Guryanova O A et al. Actin cytoskeleton remodeling by the alternatively spliced isoform of PDLIM4/RIL protein. J Biol Chem 286:26849-26859 (2011).
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
Azoreductase antibody
Cytochrome b 5 reductase antibody
DHQU antibody
DIA 4 antibody
DIA4 antibody
Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
Diaphorase (NADH/NADPH) antibody
Diaphorase 4 antibody
Dioxin inducible 1 antibody
DT diaphorase antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (HA610087) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: Saos-2 cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (HA610087) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si NQO1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"