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C10orf58 Recombinant Mouse Monoclonal Antibody [7-B7-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610088

C10orf58 Recombinant Mouse Monoclonal Antibody [7-B7-R] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

C10orf58 Recombinant Mouse Monoclonal Antibody [7-B7-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human FAM213A aa 1-229 / 229.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 25 kDa

Positive Control

HUVEC cell lysate, SK-MEL-28 cell lysate, rat skin tissue lysate, SK-MEL-28.

Conjugation

unconjugated

Clone Number

7-B7-R

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

Redox-regulatory protein FAM213A also known as peroxiredoxin-like 2 activated in M-CSF stimulated monocytes (PAMM) is a protein that in humans is encoded by the FAM213A gene.

Background References

1. Chen YF et al. miR-125b suppresses oral oncogenicity by targeting the anti-oxidative gene PRXL2A. Redox Biol. 2019 Apr

2. Oh CK et al. FAM213A is linked to prognostic significance in acute myeloid leukemia through regulation of oxidative stress and myelopoiesis. Hematol Oncol. 2020 Aug

Subcellular Location

Cytoplasm, Secreted .

UNIPROT

SwissProt: Q9BRX8 Human

SwissProt: Q6AXX6 Rat

Synonyms

Peroxiredoxin-like 2A

PRXL2A

Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes 2 Publications

Protein PAMM 2 Publications

Redox-regulatory protein FAM213A

C10orf58

FAM213A

PAMM

PRO2290

PSEC0139

Expand

Images

  • Western blot analysis of C10orf58 on different lysates with Mouse anti-C10orf58 antibody (<a href="/products/HA610088" style="font-weight: bold;text-decoration: underline;">HA610088</a>) at 1/2,000 dilution.<br /><br />Lane 1: HUVEC cell lysate (20 µg/Lane)<br />Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)<br />Lane 3: Rat skin tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 24/25 kDa<br /><br />Exposure time: 1 minute 46 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610088" style="font-weight: bold;text-decoration: underline;">HA610088</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of C10orf58 on different lysates with Mouse anti-C10orf58 antibody (HA610088) at 1/2,000 dilution.

    Lane 1: HUVEC cell lysate (20 µg/Lane)
    Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)
    Lane 3: Rat skin tissue lysate (40 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 24/25 kDa

    Exposure time: 1 minute 46 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of SK-MEL-28 cells labeling C10orf58 with Mouse anti-C10orf58 antibody (<a href="/products/HA610088" style="font-weight: bold;text-decoration: underline;">HA610088</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-C10orf58 antibody (<a href="/products/HA610088" style="font-weight: bold;text-decoration: underline;">HA610088</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SK-MEL-28 cells labeling C10orf58 with Mouse anti-C10orf58 antibody (HA610088) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-C10orf58 antibody (HA610088) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of SK-MEL-28 cells labeling C10orf58.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA610088" style="font-weight: bold;text-decoration: underline;">HA610088</a>, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Mouse IgG Secondary antibody (<a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SK-MEL-28 cells labeling C10orf58.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA610088, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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