C10orf58 Recombinant Mouse Monoclonal Antibody [7-B7-R] - BSA and Azide free

This data was developed using HA601210, the same antibody clone in a different buffer formulation.
Western blot analysis of C10orf58 on different lysates with Mouse anti-C10orf58 antibody (HA601210) at 1/2,000 dilution.

Lane 1: HUVEC cell lysate (20 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 3: Rat skin tissue lysate (40 µg/Lane)

Predicted band size: 25 kDa
Observed band size: 24/25 kDa

Exposure time: 1 minute 46 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601210) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA601210, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of SK-MEL-28 cells labeling C10orf58 with Mouse anti-C10orf58 antibody (HA601210) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-C10orf58 antibody (HA601210) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

This data was developed using HA601210, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of SK-MEL-28 cells labeling C10orf58.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA601210, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).