Skip to content

TRIM72 Recombinant Mouse Monoclonal Antibody [A1D4-R] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA610103

TRIM72 Recombinant Mouse Monoclonal Antibody [A1D4-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TRIM72 Recombinant Mouse Monoclonal Antibody [A1D4-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein with Human TRIM72 aa 50-150 / 477.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 53 kDa

Positive Control

HCT 116 cell lysate, mouse skeletal muscle tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, rat skeletal muscle tissue lysate.

Conjugation

unconjugated

Clone Number

A1D4-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).

Background References

1. Nagre N. et. al. TRIM72 modulates caveolar endocytosis in repair of lung cells. Am J Physiol Lung Cell Mol Physiol. 2016 Mar 1;310(5):L452-64.

2. Ishiwata-Endo H. et. al. Role of a TRIM72 ADP-ribosylation cycle in myocardial injury and membrane repair. JCI Insight. 2018 Nov 15;3(22). pii: 97898.

Subcellular Location

Sarcolemma, cytoplasmic vesicle membrane.

UNIPROT

SwissProt: Q6ZMU5 Human

SwissProt: Q1XH17 Mouse

SwissProt: A0JPQ4 Rat

Synonyms

LOC493829 antibody

Mg53 antibody

Mitsugumin-53 antibody

TRI72_HUMAN antibody

trim72 antibody

Tripartite motif containing 72 antibody

Tripartite motif containing protein 72 antibody

Tripartite motif-containing protein 72 antibody

Images

  • Western blot analysis of TRIM72 on different lysates with Mouse anti-TRIM72 antibody (<a href="/products/HA610103" style="font-weight: bold;text-decoration: underline;">HA610103</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate (20 µg/Lane)<br />Lane 2: Mouse skeletal muscle tissue lysate (40 µg/Lane)<br />Lane 3: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 4: Rat heart tissue lysate (40 µg/Lane)<br />Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610103" style="font-weight: bold;text-decoration: underline;">HA610103</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TRIM72 on different lysates with Mouse anti-TRIM72 antibody (HA610103) at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate (20 µg/Lane)
    Lane 2: Mouse skeletal muscle tissue lysate (40 µg/Lane)
    Lane 3: Mouse heart tissue lysate (40 µg/Lane)
    Lane 4: Rat heart tissue lysate (40 µg/Lane)
    Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610103) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"