MTERFD1 Recombinant Mouse Monoclonal Antibody [A8-A10-R] - BSA and Azide free
Catalog# HA610112
MTERFD1 Recombinant Mouse Monoclonal Antibody [A8-A10-R] - BSA and Azide free
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
MTERFD1 Recombinant Mouse Monoclonal Antibody [A8-A10-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein with Human MTERFD1 aa 29-417 / 417.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, SK-OV-3 cell lysate.
Conjugation
unconjugated
Clone Number
A8-A10-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
MTERFD1 belongs to the MTERF family which consists of four members including MTERF1, MTERF2, MTERF3 and MTERF4. Previous research showed that MTERFD1 is a mitochondrial protein that interacts with the mitochondrial DNA promoter region and decreases transcription initiation in mammalian mitochondria. This negative regulation is likely important for fine-tuning mitochondria transcription in response to physiological demands. Human mitochondrial transcription termination factor domain 1 (MTERFD1) was mutated in many types of cancer. In vivo and in vitro data indicated that MTERFD1 gene possesses oncogenic properties.
Background References
暂无
Subcellular Location
Mitochondrion.
Synonyms
CGI 12 antibody
mitochondrial antibody
Mitochondrial transcription termination factor 3 antibody
MTER1_HUMAN antibody
MTERF domain containing 1 antibody
mTERF domain containing protein 1 mitochondrial antibody
mTERF domain-containing protein 1 antibody
mTERF3 antibody
Mterfd1 antibody
Transcription termination factor 3, mitochondrial antibody
Images
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Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (HA610112) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: SK-OV-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"