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MTERFD1 Recombinant Mouse Monoclonal Antibody [A8-A10-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610112

MTERFD1 Recombinant Mouse Monoclonal Antibody [A8-A10-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

MTERFD1 Recombinant Mouse Monoclonal Antibody [A8-A10-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein with Human MTERFD1 aa 29-417 / 417.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 48 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, SK-OV-3 cell lysate, mouse kidney tissue lysate, mouse testis tissue lysate.

Conjugation

unconjugated

Clone Number

A8-A10-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

MTERFD1 belongs to the MTERF family which consists of four members including MTERF1, MTERF2, MTERF3 and MTERF4. Previous research showed that MTERFD1 is a mitochondrial protein that interacts with the mitochondrial DNA promoter region and decreases transcription initiation in mammalian mitochondria. This negative regulation is likely important for fine-tuning mitochondria transcription in response to physiological demands. Human mitochondrial transcription termination factor domain 1 (MTERFD1) was mutated in many types of cancer. In vivo and in vitro data indicated that MTERFD1 gene possesses oncogenic properties.

Background References

暂无

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: Q96E29 Human

SwissProt: Q8R3J4 Mouse

Synonyms

CGI 12 antibody

mitochondrial antibody

Mitochondrial transcription termination factor 3 antibody

MTER1_HUMAN antibody

MTERF domain containing 1 antibody

mTERF domain containing protein 1 mitochondrial antibody

mTERF domain-containing protein 1 antibody

mTERF3 antibody

Mterfd1 antibody

Transcription termination factor 3, mitochondrial antibody

Images

  • Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (<a href="/products/HA610112" style="font-weight: bold;text-decoration: underline;">HA610112</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: HEK-293 cell lysate<br />Lane 5: SH-SY5Y cell lysate<br />Lane 6: SK-OV-3 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610112" style="font-weight: bold;text-decoration: underline;">HA610112</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (HA610112) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: HEK-293 cell lysate
    Lane 5: SH-SY5Y cell lysate
    Lane 6: SK-OV-3 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 40 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (<a href="/products/HA610112" style="font-weight: bold;text-decoration: underline;">HA610112</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse kidney tissue lysate<br />Lane 2: Mouse testis tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610112" style="font-weight: bold;text-decoration: underline;">HA610112</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (HA610112) at 1/1,000 dilution.

    Lane 1: Mouse kidney tissue lysate
    Lane 2: Mouse testis tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 40 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"