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Cytokeratin 18 Recombinant Mouse Monoclonal Antibody [A4B3-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610135

Cytokeratin 18 Recombinant Mouse Monoclonal Antibody [A4B3-R] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Cytokeratin 18 Recombinant Mouse Monoclonal Antibody [A4B3-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human Cytokeratin 18 aa 1-150.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 48 kDa

Positive Control

SK-Br-3 cell lysate, A549 cell lysate, A431 cell lysate, MCF7 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

A4B3-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:2,000

Target

Function

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Background References

1. Chang YH. et. al. Elevated serum cytokeratin-18 concentration in patients with type 2 diabetes mellitus and non-alcoholic fatty liver disease. Ann Clin Biochem. 2019 Jan

2. Lai YC. et. al. Cytokeratin 18-associated Histone 3 Modulation in Hepatocellular Carcinoma: A Mini Review. Cancer Genomics Proteomics. 2017 Jul-Aug

Subcellular Location

Nucleus matrix, Cytoplasm, perinuclear region, Nucleus, nucleolus.

UNIPROT

SwissProt: P05783 Human

SwissProt: P05784 Mouse

SwissProt: Q5BJY9 Rat

Synonyms

Cell proliferation inducing gene 46 protein antibody

Cell proliferation inducing protein 46 antibody

Cell proliferation-inducing gene 46 protein antibody

CK 18 antibody

CK-18 antibody

CK18 antibody

CYK 18 antibody

CYK18 antibody

Cytokeratin 18 antibody

Cytokeratin endo B antibody

Expand

Images

  • Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/1,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: A431 cell lysate (20 µg/Lane)<br />Lane 4: MCF7 cell lysate (20 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 6: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 48 kDa<br />Observed band size: 40-50 kDa<br /><br />Exposure time: Lane 1-4: 11 seconds; Lane 5-6: 2 minutes 24 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (HA610135) at 1/1,000 dilution.

    Lane 1: SK-Br-3 cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: A431 cell lysate (20 µg/Lane)
    Lane 4: MCF7 cell lysate (20 µg/Lane)
    Lane 5: Mouse liver tissue lysate (40 µg/Lane)
    Lane 6: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 48 kDa
    Observed band size: 40-50 kDa

    Exposure time: Lane 1-4: 11 seconds; Lane 5-6: 2 minutes 24 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610135) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 18 antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 18 antibody (HA610135) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610135) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Cytokeratin 18 antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Cytokeratin 18 antibody (HA610135) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610135) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Cytokeratin 18 antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610135" style="font-weight: bold;text-decoration: underline;">HA610135</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Cytokeratin 18 antibody (HA610135) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610135) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"