Integrin alpha 3 Recombinant Mouse Monoclonal Antibody [A7H2-R] - BSA and Azide free
Catalog# HA610148
Integrin alpha 3 Recombinant Mouse Monoclonal Antibody [A7H2-R] - BSA and Azide free
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
Integrin alpha 3 Recombinant Mouse Monoclonal Antibody [A7H2-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Integrin alpha-3 aa 51-250 / 1,051.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 117 kDa
Positive Control
U-87 MG cell lysate, HeLa cell lysate, HUVEC cell lysate, A431 cell lysate, human kidney tissue lysate, U-87 MG.
Conjugation
unconjugated
Clone Number
A7H2-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
Integrin alpha-3 is a protein that in humans is encoded by the ITGA3 gene. ITGA3 is an integrin alpha subunit. Together with beta-1 subunit, it makes up half of the α3β1 integrin duplex that plays a role in neural migration and corticogenesis, acted upon by such factors as netrin-1 and reelin. ITGA3 encodes the integrin alpha 3 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 ('VLA-3'), very common antigen 2 ('VCA-2'), extracellular matrix receptor 1 ('ECMR1'), and galactoprotein b3 ('GAPB3').
Background References
1. Zhang H. et. al. ITGA3 interacts with VASP to regulate stemness and epithelial-mesenchymal transition of breast cancer cells. Gene. 2020 Apr
2. Tian L. et. al. MicroRNA-199a-5p suppresses cell proliferation, migration and invasion by targeting ITGA3 in colorectal cancer. Mol Med Rep. 2020 Sep
Subcellular Location
Cell membrane, invadopodium membrane, filopodium membrane.
UNIPROT
Synonyms
AA407068 antibody
Antigen identified by monoclonal antibody J143 antibody
CD49 antigen like family member C antibody
CD49 antigen-like family member C antibody
CD49c antibody
CD49c antigen antibody
FLJ34631 antibody
FLJ34704 antibody
FRP 2 antibody
FRP-2 antibody
ExpandImages
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Western blot analysis of Integrin alpha 3 on different lysates with Mouse anti-Integrin alpha 3 antibody (HA610148) at 1/1,000 dilution.
Lane 1: U-87 MG cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: HUVEC cell lysate (10 µg/Lane)
Lane 4: A431 cell lysate (10 µg/Lane)
Lane 5: Human kidney tissue lysate (20 µg/Lane)
Predicted band size: 117 kDa
Observed band size: 130 kDa
Exposure time: 28 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610148) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of U-87 MG cells labeling Integrin alpha 3 with Mouse anti-Integrin alpha 3 antibody (HA610148) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Integrin alpha 3 antibody (HA610148) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Integrin alpha 3 Mouse Monoclonal Antibody [A7H2]
Application: WB,IF-Cell
Reactivity: Human
Conjugate: unconjugated
Integrin alpha 3 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Rat
Conjugate: unconjugated
