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Glucosidase 2 subunit beta Recombinant Mouse Monoclonal Antibody [A6H9-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610151

Glucosidase 2 subunit beta Recombinant Mouse Monoclonal Antibody [A6H9-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Glucosidase 2 subunit beta Recombinant Mouse Monoclonal Antibody [A6H9-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human Glucosidase 2 subunit beta aa 51-250.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 59 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate.

Conjugation

unconjugated

Clone Number

A6H9-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity). This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.

Background References

1. Huang R. et. al. PRKCSH Alternative Splicing Involves in Silica-Induced Expression of Epithelial-Mesenchymal Transition Markers and Cell Proliferation. Dose Response. 2020 May

2. Shin GC. et. al. PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway. Nat Commun. 2019 Jul

Subcellular Location

Endoplasmic reticulum.

UNIPROT

SwissProt: P14314 Human

Synonyms

80K-H protein antibody

AGE-binding receptor 2 antibody

AGE-R2 antibody

G19P1 antibody

GLU2B_HUMAN antibody

Glucosidase 2 subunit beta antibody

Glucosidase II beta subunit antibody

Glucosidase II subunit beta antibody

Hepatocystin antibody

PCLD antibody

Expand

Images

  • This data was developed using <a href="/products/HA601276" style="font-weight: bold;text-decoration: underline;">HA601276</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (<a href="/products/HA601276" style="font-weight: bold;text-decoration: underline;">HA601276</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: Daudi cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: A431 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601276" style="font-weight: bold;text-decoration: underline;">HA601276</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA601276, the same antibody clone in a different buffer formulation.
    Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (HA601276) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Daudi cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: A431 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 80 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601276) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />This data was developed using <a href="/products/HA601276" style="font-weight: bold;text-decoration: underline;">HA601276</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (<a href="/products/HA601276" style="font-weight: bold;text-decoration: underline;">HA601276</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601276" style="font-weight: bold;text-decoration: underline;">HA601276</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    This data was developed using HA601276, the same antibody clone in a different buffer formulation.
    Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (HA601276) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 75 kDa

    Exposure time: 15 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601276) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"