eIF-6 Recombinant Mouse Monoclonal Antibody [A6A2-R] - BSA and Azide free
Catalog# HA610167
eIF-6 Recombinant Mouse Monoclonal Antibody [A6A2-R] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
eIF-6 Recombinant Mouse Monoclonal Antibody [A6A2-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human eIF-6 aa 1-200.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 27 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
A6A2-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis.
Background References
1. Liu Z. et. al. Long noncoding RNA PICSAR/miR-588/EIF6 axis regulates tumorigenesis of hepatocellular carcinoma by activating PI3K/AKT/mTOR signaling pathway. Cancer Sci. 2020 Nov
2. Pesce E. et. al. Discovery and Preliminary Characterization of Translational Modulators that Impair the Binding of eIF6 to 60S Ribosomal Subunits. Cells. 2020 Jan
Subcellular Location
Cytoplasm, Nucleus, nucleolus.
Synonyms
b(2)gcn antibody
B(2)GCN homolog antibody
B4 integrin interactor antibody
Binding protein of beta-4 integrin antibody
CAB antibody
eIF-6 antibody
EIF3A antibody
EIF6 antibody
Eukaryotic translation initiation factor 3A antibody
Eukaryotic translation initiation factor 6 antibody
ExpandImages
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This data was developed using HA601294, the same antibody clone in a different buffer formulation.
Western blot analysis of eIF-6 on different lysates with Mouse anti-eIF-6 antibody (HA601294) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Rat testis tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601294) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"