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Junctophilin-2 Recombinant Mouse Monoclonal Antibody [3-2-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610170

Junctophilin-2 Recombinant Mouse Monoclonal Antibody [3-2-R] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Junctophilin-2 Recombinant Mouse Monoclonal Antibody [3-2-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Junctophilin-2 aa 1-50 / 696.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 74 kDa

Positive Control

Rat skeletal muscle tissue lysates, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.

Conjugation

unconjugated

Clone Number

3-2-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Junctophilin-2 (JPH2) is a cardiac specific member of the junctophilins, a newly characterized family of junctional membrane complex proteins important in physically approximating the plasmalemmal L-type calcium channel and the sarcoplasmic reticulum ryanodine receptor for calcium-induced calcium release. It acts probably by anchoring the plasma membrane and endoplasmic reticulum. JPH2 contributes to the construction of skeletal muscle triad junctions, and plays an essential role in heart development.

Background References

1. Takeshima, H., et al. 2000. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell 6: 11-22.

2. Nishi, M., et al. 2003. Coexpression of Junctophilin type 3 and type 4 in brain. Brain Res. Mol. Brain Res. 118: 102-110.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q9BR39 Human

SwissProt: Q9ET78 Mouse

SwissProt: Q2PS20 Rat

Synonyms

FLJ40969 antibody

JP-2 antibody

JP2 antibody

Jph2 antibody

JPH2_HUMAN antibody

Junctophilin 2 antibody

Junctophilin type 2 antibody

Junctophilin-2 antibody

OTTHUMP00000031651 antibody

OTTHUMP00000031652 antibody

Images

  • Western blot analysis of Junctophilin-2 on rat skeletal muscle tissue lysates with Mouse anti-Junctophilin-2 antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Junctophilin-2 on rat skeletal muscle tissue lysates with Mouse anti-Junctophilin-2 antibody (HA610170) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610170) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (HA610170) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610170) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (HA610170) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610170) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610170" style="font-weight: bold;text-decoration: underline;">HA610170</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (HA610170) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610170) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"