KIAA0100 Recombinant Mouse Monoclonal Antibody [A0-F3-R] - BSA and Azide free
Catalog# HA610189
KIAA0100 Recombinant Mouse Monoclonal Antibody [A0-F3-R] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
KIAA0100 Recombinant Mouse Monoclonal Antibody [A0-F3-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human KIAA0100 aa 1,947-2,235 / 2,235.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 254 kDa
Positive Control
MDA-MB-231 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, HeLa cell lysate, A431 cell lysate, PC-3M cell lysate, LNCaP cell lysate, HeLa, human breast cancer tissue, human kidney tissue, human pancreas tissue.
Conjugation
unconjugated
Clone Number
A0-F3-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Breast cancer-overexpressed gene 1 protein (KIAA0100) is a glucoprotein composed of 2,235 amino acids. KIAA0100 has four isforms (254kDa,250kDa,45kDa,25kDa).KIAA0100 is expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells. This gene was initially characterized in human as having high expression levels in breast carcinomas and breast cancer cell lines. This gene also has increased expression in prostrate cancer cells relative to normal prostrate tissues. Expression of this gene is negatively regulated by direct binding of the microRNA miR-195 to its 3' UTR. miR-195 has been shown to modulate the invasiveness of prostrate cancer cells and xenograft metastases by downgrading expression of this gene. In mouse, the protein encoded by this gene was identified as an antigen on acute monocytic leukemia cells. In human, alternative splicing results in multiple transcript variants encoding distinct isoforms; some of these isoforms are predicted to contain an RNA pol II promoter FMP27 protein domain and a Golgi-body-localization APT1 domain.
Background References
1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)
2. "Serological identification of immunogenic antigens in acute monocytic leukemia."Chen G., Zhang W.G., Cao X.M., Li F.Y., Liu X.P., Yao L.B.Leuk. Res. 29:503-509(2005)
Subcellular Location
Cell membrane, Endoplasmic reticulum membrane, Mitochondrion membrane.
UNIPROT
Synonyms
Antigen MLAA-22 antibody
BCOX antibody
BCOX1 antibody
Breast cancer-overexpressed gene 1 protein antibody
CT101 antibody
DKFZp686M0843 antibody
K0100_HUMAN antibody
KIAA0100 antibody
MGC111488 antibody
MGC134981 antibody
ExpandImages
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This data was developed using HA601317, the same antibody clone in a different buffer formulation.
Western blot analysis of KIAA0100 on different lysates with Mouse anti-KIAA0100 antibody (HA601317) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: SK-Br-3 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: A431 cell lysate
Lane 6: PC-3M cell lysate
Lane 7: LNCaP cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 254 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA601317, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa cells labeling KIAA0100 with Mouse anti-KIAA0100 antibody (HA601317) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-KIAA0100 antibody (HA601317) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
This data was developed using HA601317, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-KIAA0100 antibody (HA601317) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601317) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601317, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-KIAA0100 antibody (HA601317) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601317) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601317, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-KIAA0100 antibody (HA601317) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601317) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"