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Claudin 7 Recombinant Mouse Monoclonal Antibody [6-G3-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610206

Claudin 7 Recombinant Mouse Monoclonal Antibody [6-G3-R] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Claudin 7 Recombinant Mouse Monoclonal Antibody [6-G3-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Claudin 7 aa 162-211 / 211.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, IF-Tissue, IP

Molecular Weight

Predicted band size: 22 kDa

Positive Control

SW480 cell lysate, MCF7 cell lysate, LNCaP cell lysate, Caco-2 cell lysate, A431 cell lysate, mouse colon tissue lysate, rat colon tissue lysate, PC-12, human kidney tissue, human colon tissue, mouse colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

6-G3-R

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:5,000

  • IF-Tissue

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins.Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells. It is expressed in kidney, lung and prostate. Claudin-7 is down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

Background References

1. Xiao Y et al. Matrix metalloproteinase 7 contributes to intestinal barrier dysfunction by degrading tight junction protein Claudin-7. Front Immunol. 2022 Oct

2. Ahlswede L et al. Human Claudin-7 cis-Interactions Are Not Crucial for Membrane-Membrane (Trans-) Interactions. Front Mol Biosci. 2022 Jun

Subcellular Location

Cell membrane, Basolateral cell membrane, Cell junction, tight junction.

UNIPROT

SwissProt: O95471 Human

SwissProt: Q9Z261 Mouse

SwissProt: Q9Z1L1 Rat

Synonyms

CEPTR L2 antibody

CEPTRL 2 antibody

CEPTRL2 antibody

Claudin 1 antibody

Claudin 9 antibody

Claudin-7 antibody

Claudin1 antibody

Claudin7 antibody

Claudin9 antibody

CLD7_HUMAN antibody

Expand

Images

  • Western blot analysis of Claudin 7 on different lysates with Mouse anti-Claudin 7 antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: LNCaP cell lysate (20 µg/Lane)<br />Lane 4: Caco-2 cell lysate (20 µg/Lane)<br />Lane 5: A431 cell lysate (20 µg/Lane)<br />Lane 6: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 7: Rat colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 22 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Claudin 7 on different lysates with Mouse anti-Claudin 7 antibody (HA610206) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: LNCaP cell lysate (20 µg/Lane)
    Lane 4: Caco-2 cell lysate (20 µg/Lane)
    Lane 5: A431 cell lysate (20 µg/Lane)
    Lane 6: Mouse colon tissue lysate (40 µg/Lane)
    Lane 7: Rat colon tissue lysate (40 µg/Lane)

    Predicted band size: 22 kDa
    Observed band size: 20 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610206) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of PC-12 cells labeling Claudin 7 with Mouse anti-Claudin 7 antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Claudin 7 antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling Claudin 7 with Mouse anti-Claudin 7 antibody (HA610206) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Claudin 7 antibody (HA610206) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Claudin 7 antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Claudin 7 antibody (HA610206) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610206) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Claudin 7 antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Claudin 7 antibody (HA610206) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610206) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-Claudin 7 antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-Claudin 7 antibody (HA610206) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610206) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-Claudin 7 antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-Claudin 7 antibody (HA610206) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610206) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Claudin 7 was immunoprecipitated from 0.2 mg A431 cell lysate with <a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a> at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A431 cell lysate (input)<br />Lane 2: <a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a> IP in A431 cell lysate<br />Lane 3: Mouse IgG instead of <a href="/products/HA610206" style="font-weight: bold;text-decoration: underline;">HA610206</a> in A431 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 2 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Claudin 7 was immunoprecipitated from 0.2 mg A431 cell lysate with HA610206 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA610206 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A431 cell lysate (input)
    Lane 2: HA610206 IP in A431 cell lysate
    Lane 3: Mouse IgG instead of HA610206 in A431 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 2 minutes; ECL: K1801

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