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SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610228

SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47] - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

PSH10-47

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

Target

Function

Vesicular inhibitory amino acid transporter is a protein that in humans is encoded by the SLC32A1 gene. The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.

Background References

1. Heron SE et al. Association of SLC32A1 Missense Variants With Genetic Epilepsy With Febrile Seizures Plus. Neurology. 2021 May

2. Guerrini R et al. SLC32A1: One More Gene Contributing to the Solution of the Genetic Generalized Epilepsies Mystery. Neurology. 2021 May

Subcellular Location

Cytoplasmic vesicle membrane, Presynapse.

UNIPROT

SwissProt: O35633 Mouse

SwissProt: O35458 Rat

Synonyms

bA122O1.1 antibody

GABA and glycine transporter antibody

hVIAAT antibody

SLC32A 1 antibody

Slc32a1 antibody

solute carrier family 32 (GABA vesicular transporter) member 1 antibody

Solute carrier family 32 member 1 antibody

Vesicular GABA Amino Acid Transporter antibody

Vesicular GABA transporter antibody

Vesicular inhibitory amino acid transporter antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (SLC32A1 / VGAT, <a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>, green); 1:500 (Parvalbumin, <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500 (SLC32A1 / VGAT, HA610228, green); 1:500 (Parvalbumin, ET1703-15, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (SLC32A1 / VGAT, <a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>, green); 1:500 (Parvalbumin, <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500 (SLC32A1 / VGAT, HA610228, green); 1:500 (Parvalbumin, ET1703-15, red)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA610228) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA610228) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA610228) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610228" style="font-weight: bold;text-decoration: underline;">HA610228</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA610228) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"