VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610250
VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Rat IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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Mouse
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Species independent
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Pig
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unconjugated
Overview
Product Name
VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within N terminal Mouse VAChT.
Species Reactivity
Mouse (Predicted: Species independent, Pig)
Predicted species support after-sales service
Validated Applications
IHC-Fr
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
Mouse brain tissue.
Conjugation
unconjugated
Clone Number
PSH10-50
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:500
Target
Function
The Vesicular acetylcholine transporter (VAChT) is a neurotransmitter transporter which is responsible for loading acetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene, located within the first intron of the choline acetyltransferase gene. VAChT is able to transport ACh into vesicles by relying on an exchange between protons (H+) that were previously pumped into the vesicle diffusing out, thus acting as an antiporter. ACh molecules are then carried into the vesicle by the action of exiting protons. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase.
Background References
1. Zhang Y et al. Structural insights into VAChT neurotransmitter recognition and inhibition. Cell Res. 2024 Sep
2. Pergolizzi S et al. Expression of VAChT and 5-HT in Ulcerative colitis dendritic cells. Acta Histochem. 2021 May
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
UNIPROT
Synonyms
AChR antibody
MGC12716 antibody
rVAT antibody
Slc18a3 antibody
Solute carrier family 18 (vesicular acetylcholine) member 3 antibody
Solute carrier family 18 (vesicular monoamine) member 3 antibody
Solute carrier family 18 member 3 antibody
VAChT antibody
VACHT_HUMAN antibody
Vesicular acetylcholine transporter antibody
Images
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Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"