HLA Class I Recombinant Mouse Monoclonal Antibody [PSH12-15] - BSA and Azide free
Catalog# HA610264
HLA Class I Recombinant Mouse Monoclonal Antibody [PSH12-15] - BSA and Azide free
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IF-Cell
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FC
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Human
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HA601424
含抗保成分
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unconjugated
Overview
Product Name
HLA Class I Recombinant Mouse Monoclonal Antibody [PSH12-15] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within N terminal Human HLA Class I.
Species Reactivity
Human
Validated Applications
IF-Cell, FC
Molecular Weight
Predicted band size: 41 kDa
Positive Control
Jurkat.
Conjugation
unconjugated
Clone Number
PSH12-15
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
MHC class I molecules are one of two primary classes of major histocompatibility complex (MHC) molecules (the other being MHC class II) and are found on the cell surface of all nucleated cells in the bodies of vertebrates. They also occur on platelets, but not on red blood cells. Their function is to display peptide fragments of proteins from within the cell to cytotoxic T cells; this will trigger an immediate response from the immune system against a particular non-self antigen displayed with the help of an MHC class I protein. Because MHC class I molecules present peptides derived from cytosolic proteins, the pathway of MHC class I presentation is often called cytosolic or endogenous pathway. In humans, the HLAs corresponding to MHC class I are HLA-A, HLA-B, and HLA-C.
Background References
1. Bettens F et al. Regulation of HLA class I expression by non-coding gene variations. PLoS Genet. 2022 Jun
2. Pei R et al. Prognostic Value of HLA Class I in Patients with Hepatocellular Carcinoma. Clin Lab. 2022 Jun
Subcellular Location
Cell membrane, Endoplasmic reticulum membrane.
Synonyms
Antigen Presenting Molecule antibody
Cw*1701 antibody
D6S204 antibody
FLJ27082 antibody
HLA A antibody
HLA B antibody
HLA C antibody
HLA class 1 A antibody
HLA class 1 B antibody
HLA class 1 C antibody
ExpandImages
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Immunocytochemistry analysis of Jurkat cells labeling HLA Class I with Mouse anti-HLA Class I antibody (HA610264) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HLA Class I antibody (HA610264) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
Flow cytometric analysis of K-562 (left, negative) and Jurkat (right, positive) cells labeling HLA Class I.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA610264, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"