GAD65 Recombinant Antibody [PSH06-80] - Mouse IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610289
GAD65 Recombinant Antibody [PSH06-80] - Mouse IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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IHC-P
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WB
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Mouse
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Rat
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unconjugated
Overview
Product Name
GAD65 Recombinant Antibody [PSH06-80] - Mouse IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, IHC-P, WB
Target Molecular Weight
Predicted band size: 65 kDa
Positive Control
Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH06-80
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Background References
1. Budhram A et al. Clinical spectrum of high-titre GAD65 antibodies. J Neurol Neurosurg Psychiatry. 2021 Feb
2. Budhram A et al. Positive Predictive Value of Anti-GAD65 ELISA Cut-Offs for Neurological Autoimmunity. Can J Neurol Sci. 2023 Sep
Subcellular Location
Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane.
Synonyms
65 kDa glutamic acid decarboxylase antibody
DCE 2 antibody
DCE2 antibody
DCE2_HUMAN antibody
GAD 2 antibody
GAD 65 antibody
GAD-2 antibody
GAD-65 antibody
Gad2 antibody
Glutamate decarboxylase 2 (pancreas) antibody
ExpandImages
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This data was developed using HA601455, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
This data was developed using HA601455, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601455, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601455, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601455, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601455, the same antibody clone in a different buffer formulation.
Western blot analysis of GAD65 on different lysates with Mouse anti-GAD65 antibody (HA601455) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601455) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"