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Dopamine Transporter Recombinant Antibody [PSH10-49] - Mouse IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610310

Dopamine Transporter Recombinant Antibody [PSH10-49] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Dopamine Transporter Recombinant Antibody [PSH10-49] - Mouse IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P

Target Molecular Weight

Predicted band size: 69 kDa

Positive Control

Mouse brain (striatum) tissue, rat brain (striatum) tissue.

Conjugation

unconjugated

Clone Number

PSH10-49

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The dopamine transporter (also dopamine active transporter, DAT, SLC6A3) is a membrane-spanning protein that pumps the neurotransmitter dopamine out of the synaptic cleft back into cytosol. In the cytosol, other transporters sequester the dopamine into vesicles for storage and later release. Dopamine reuptake via DAT provides the primary mechanism through which dopamine is cleared from synapses, although there may be an exception in the prefrontal cortex, where evidence points to a possibly larger role of the norepinephrine transporter.DAT is implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder, bipolar disorder, clinical depression, alcoholism, and substance use disorder. The gene that encodes the DAT protein is located on human chromosome 5, consists of 15 coding exons, and is roughly 64 kbp long. Evidence for the associations between DAT and dopamine related disorders has come from a type of genetic polymorphism, known as a VNTR, in the DAT gene (DAT1), which influences the amount of protein expressed.

Background References

1. Savchenko A et al. Dopamine Transporter Deficient Rodents: Perspectives and Limitations for Neuroscience. Biomolecules. 2023 May

2. Ng J et al. Dopamine Transporter Deficiency Syndrome (DTDS): Expanding the Clinical Phenotype and Precision Medicine Approaches. Cells. 2023 Jun

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q61327 Mouse

SwissProt: P23977 Rat

Synonyms

DA transporter antibody

DAT 1 antibody

DAT antibody

DAT1 antibody

Dopamine transporter 1 antibody

Dopamine transporter antibody

PKDYS antibody

SC6A3_HUMAN antibody

SLC6A3 antibody

Sodium dependent dopamine transporter antibody

Expand

Images

  • This data was developed using <a href="/products/HA601476" style="font-weight: bold;text-decoration: underline;">HA601476</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain (striatum)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    This data was developed using HA601476, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: brain (striatum)

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601476" style="font-weight: bold;text-decoration: underline;">HA601476</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse brain (striatum) tissue with Mouse anti-Dopamine Transporter antibody (<a href="/products/HA601476" style="font-weight: bold;text-decoration: underline;">HA601476</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601476" style="font-weight: bold;text-decoration: underline;">HA601476</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601476, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse brain (striatum) tissue with Mouse anti-Dopamine Transporter antibody (HA601476) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601476) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601476" style="font-weight: bold;text-decoration: underline;">HA601476</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat brain (striatum) tissue with Mouse anti-Dopamine Transporter antibody (<a href="/products/HA601476" style="font-weight: bold;text-decoration: underline;">HA601476</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601476" style="font-weight: bold;text-decoration: underline;">HA601476</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601476, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat brain (striatum) tissue with Mouse anti-Dopamine Transporter antibody (HA601476) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601476) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"