HA tag Recombinant Mouse Monoclonal Antibody - BSA and Azide free [2-G10] (HA610320)
Catalog# HA610320
Mouse Monoclonal to HA tag
Tested for
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WB
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IHC-P
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IF-Cell
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HA tag on different lysates with Mouse anti-HA tag antibody (HA610320) at 1/10,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610320) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610320) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.