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TPH2 Recombinant Antibody [PSH04-89] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610322

TPH2 Recombinant Antibody [PSH04-89] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TPH2 Recombinant Antibody [PSH04-89] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein with Human THP2 aa 1-490.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 56 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH04-89

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200-1:500

  • WB

  • 1:5,000

Target

Function

Tryptophan hydroxylase 2 (TPH2) is an isozyme of tryptophan hydroxylase found in vertebrates. In humans, TPH2 is primarily expressed in the serotonergic neurons of the brain, with the highest expression in the raphe nucleus of the midbrain. Until the discovery of TPH2 in 2003, serotonin levels in the central nervous system were believed to be regulated by serotonin synthesis in peripheral tissues, in which tryptophan hydroxylase is the dominant form.

Background References

1. Zaniewska M et al. Tph2 Gene Expression Defines Ethanol Drinking Behavior in Mice. Cells. 2022 Mar

2. Liu H et al. TPH2 in the Dorsal Raphe Nuclei Regulates Energy Balance in a Sex-Dependent Manner. Endocrinology. 2021 Jan

Subcellular Location

Cytosol, neuron projection.

UNIPROT

SwissProt: Q8CGV2 Mouse

SwissProt: Q8CGU9 Rat

Synonyms

MGC138871 antibody

ADHD7 antibody

FLJ37295 antibody

MGC138872 antibody

Neuronal tryptophan hydroxylase antibody

NTPH antibody

TPH 2 antibody

Tph2 antibody

TPH2_HUMAN antibody

Tryptophan 5-hydroxylase 2 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Raphe nucleus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Raphe nucleus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TPH2 on different lysates with Guinea pig anti-TPH2 antibody (<a href="/products/HA610322" style="font-weight: bold;text-decoration: underline;">HA610322</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse kidney tissue lysate (negative)<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat kidney tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610322" style="font-weight: bold;text-decoration: underline;">HA610322</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TPH2 on different lysates with Guinea pig anti-TPH2 antibody (HA610322) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse kidney tissue lysate (negative)
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat kidney tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 50 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610322) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"