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Doublecortin Recombinant Antibody [JJ0959] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610326

Doublecortin Recombinant Antibody [JJ0959] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Doublecortin Recombinant Antibody [JJ0959] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human Doublecortin aa 22-71 / 365.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 41 kDa

Positive Control

SH-SY5Y cell lysate, Mouse brain (P0) tissue lysate, Mouse brain (P60) tissue lysate.

Conjugation

unconjugated

Clone Number

JJ0959

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1::200

  • WB

  • 1:10,000

Target

Function

Neuronal migration protein doublecortin, also known as doublin or lissencephalin-X is a protein that in humans is encoded by the DCX gene. Doublecortin (DCX) is a microtubule-associated protein expressed by neuronal precursor cells and immature neurons in embryonic and adult cortical structures. Neuronal precursor cells begin to express DCX while actively dividing, and their neuronal daughter cells continue to express DCX for 2–3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express NeuN, a neuronal marker.

Background References

1. Fu X et al. Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking. Elife. 2022 Dec

2. Li YN et al. Doublecortin-Expressing Neurons in Human Cerebral Cortex Layer II and Amygdala from Infancy to 100 Years Old. Mol Neurobiol. 2023 Jun

Subcellular Location

Cytoplasm, Cell projection, neuron projection.

UNIPROT

SwissProt: O43602 Human

SwissProt: O88809 Mouse

SwissProt: Q9ESI7 Rat

Synonyms

DBCN antibody

Dbct antibody

DC antibody

DCX antibody

DCX_HUMAN antibody

Doublecortex antibody

Doublin antibody

FLJ51296 antibody

Lis X antibody

Lis-X antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: hippocampus

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Doublecortin on different lysates with Guinea pig anti-Doublecortin antibody (<a href="/products/HA610326" style="font-weight: bold;text-decoration: underline;">HA610326</a>) at 1/10,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (15 µg/Lane)<br />Lane 2: A549 cell lysate (negative) (15 µg/Lane)<br />Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane)<br />Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane)<br />Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41/45 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610326" style="font-weight: bold;text-decoration: underline;">HA610326</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Doublecortin on different lysates with Guinea pig anti-Doublecortin antibody (HA610326) at 1/10,000 dilution.

    Lane 1: SH-SY5Y cell lysate (15 µg/Lane)
    Lane 2: A549 cell lysate (negative) (15 µg/Lane)
    Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane)
    Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane)
    Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41/45 kDa

    Exposure time: 14 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610326) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"