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Doublecortin Recombinant Antibody [JJ0959] - Mouse IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610327

Doublecortin Recombinant Antibody [JJ0959] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Doublecortin Recombinant Antibody [JJ0959] - Mouse IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human Doublecortin aa 22-71 / 365.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Target Molecular Weight

Predicted band size: 41 kDa

Positive Control

Mouse hippocampus tissue, rat hippocampus tissue, SH-SY5Y cell lysate, Mouse brain (P0) tissue lysate, Mouse brain (P60) tissue lysate.

Conjugation

unconjugated

Clone Number

JJ0959

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

  • WB

  • 1:10,000

Target

Function

Neuronal migration protein doublecortin, also known as doublin or lissencephalin-X is a protein that in humans is encoded by the DCX gene. Doublecortin (DCX) is a microtubule-associated protein expressed by neuronal precursor cells and immature neurons in embryonic and adult cortical structures. Neuronal precursor cells begin to express DCX while actively dividing, and their neuronal daughter cells continue to express DCX for 2–3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express NeuN, a neuronal marker.

Background References

1. Fu X et al. Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking. Elife. 2022 Dec

2. Li YN et al. Doublecortin-Expressing Neurons in Human Cerebral Cortex Layer II and Amygdala from Infancy to 100 Years Old. Mol Neurobiol. 2023 Jun

Subcellular Location

Cytoplasm, Cell projection, neuron projection.

UNIPROT

SwissProt: O43602 Human

SwissProt: O88809 Mouse

SwissProt: Q9ESI7 Rat

Synonyms

DBCN antibody

Dbct antibody

DC antibody

DCX antibody

DCX_HUMAN antibody

Doublecortex antibody

Doublin antibody

FLJ51296 antibody

Lis X antibody

Lis-X antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-Doublecortin antibody (<a href="/products/HA610327" style="font-weight: bold;text-decoration: underline;">HA610327</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610327" style="font-weight: bold;text-decoration: underline;">HA610327</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-Doublecortin antibody (HA610327) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610327) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-Doublecortin antibody (<a href="/products/HA610327" style="font-weight: bold;text-decoration: underline;">HA610327</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610327" style="font-weight: bold;text-decoration: underline;">HA610327</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-Doublecortin antibody (HA610327) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610327) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Doublecortin on different lysates with Mouse anti-Doublecortin antibody (<a href="/products/HA610327" style="font-weight: bold;text-decoration: underline;">HA610327</a>) at 1/10,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (15 µg/Lane)<br />Lane 2: A549 cell lysate (negative) (15 µg/Lane)<br />Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane)<br />Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane)<br />Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41/45 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610327" style="font-weight: bold;text-decoration: underline;">HA610327</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Doublecortin on different lysates with Mouse anti-Doublecortin antibody (HA610327) at 1/10,000 dilution.

    Lane 1: SH-SY5Y cell lysate (15 µg/Lane)
    Lane 2: A549 cell lysate (negative) (15 µg/Lane)
    Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane)
    Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane)
    Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41/45 kDa

    Exposure time: 14 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610327) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"