MCM2 Recombinant Antibody [PD00-90] - Mouse IgG1 (Chimeric) - BSA and Azide free (HA610350)
Catalog# HA610350
to MCM2
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MCM2 on different lysates with Mouse anti-MCM2 antibody (HA610350) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 130 kDa
Exposure time: 11 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610350) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 130 kDa
Exposure time: 11 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610350) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.