S100 beta Recombinant Antibody [SC57-02] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610367
S100 beta Recombinant Antibody [SC57-02] - Rat IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
S100 beta Recombinant Antibody [SC57-02] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within C-terminal human S100 beta.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, IHC-P
Target Molecular Weight
Predicted band size: 11 kDa
Positive Control
Mouse hippocampus tissue, rat hippocampus tissue.
Conjugation
unconjugated
Clone Number
SC57-02
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| IHC-P | IHC-Fr | |
|---|---|---|
| mouse |
|
|
| rat |
|
|
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
S100 calcium-binding protein B (S100B) is a protein of the S100 protein family. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker.
Background References
1. Duan K et al. S100-beta aggravates spinal cord injury via activation of M1 macrophage phenotype. J Musculoskelet Neuronal Interact. 2021 Sep
2. Hanin A et al. Neuron Specific Enolase, S100-beta protein and progranulin as diagnostic biomarkers of status epilepticus. J Neurol. 2022 Jul
Subcellular Location
Cytoplasm, Nucleus, Secreted.
Synonyms
NEF antibody
Protein S100 B antibody
Protein S100-B antibody
S 100 calcium binding protein beta chain antibody
S 100 protein beta chain antibody
S-100 protein beta chain antibody
S-100 protein subunit beta antibody
S100 antibody
S100 calcium binding protein beta (neural) antibody
S100 calcium-binding protein B antibody
ExpandImages
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This data was developed using HA601552, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA601552, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500 (S100 beta, HA601552, Rat, red); 1/200 (SOX2, HA601499, Chicken, green)
Antigen retrieval: Not required -
This data was developed using HA601552, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-S100 beta antibody (HA601552) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601552) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601552, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-S100 beta antibody (HA601552) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601552) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"