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Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610392

Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IF-Tissue

Molecular Weight

Predicted band size: 33 kDa

Positive Control

Human brain tissue, mouse brain tissue, rat brain tissue,

Conjugation

unconjugated

Clone Number

JF0943

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200

  • IF-Tissue

  • 1:500

Target

Function

Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system myelin. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin sheath. Mitogen-activated protein kinases modulate MBP phosphorylation during myelinogenesis and in the demyelinating disease multiple sclerosis. MBP phosphorylation is regulated by high-frequency stimulation but not low-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus. It is proposed that during periods of increased neuronal activity, calcium activates axonal nitric oxide synthase, which generates the intercellular messengers nitric oxide and superoxide and regulates the phosphorylation state of MBP by MAPK.

Background References

1. Martinsen V et al. Multiple sclerosis and myelin basic protein: insights into protein disorder and disease. Amino Acids. 2022 Jan

2. Bagheri H et al. Myelin basic protein mRNA levels affect myelin sheath dimensions, architecture, plasticity, and density of resident glial cells. Glia. 2024 Oct

Subcellular Location

Myelin membrane, Nucleus.

UNIPROT

SwissProt: P02686 Human

SwissProt: P04370 Mouse

SwissProt: P02688 Rat

Synonyms

GDB antibody

Golli MBP antibody

Golli MBP

myelin basic protein antibody

Hemopoietic MBP antibody

HMBPR antibody

HUGO antibody

MBP antibody

MBP_CAVPO antibody

MBP_HUMAN antibody

Expand

Images

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebral cortex<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA610392" style="font-weight: bold;text-decoration: underline;">HA610392</a>, 1/200, overnight at 4℃.<br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488, <a href="/products/HA1139" style="font-weight: bold;text-decoration: underline;">HA1139</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebral cortex
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA610392, 1/200, overnight at 4℃.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Hippocampus<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA610392" style="font-weight: bold;text-decoration: underline;">HA610392</a>, 1/200, overnight at 4℃.<br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488, <a href="/products/HA1139" style="font-weight: bold;text-decoration: underline;">HA1139</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Hippocampus
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA610392, 1/200, overnight at 4℃.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Human<br />Tissue: Brain<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA610392" style="font-weight: bold;text-decoration: underline;">HA610392</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488, <a href="/products/HA1139" style="font-weight: bold;text-decoration: underline;">HA1139</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610392, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Mouse<br />Tissue: Cerebral cortex<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA610392" style="font-weight: bold;text-decoration: underline;">HA610392</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488, <a href="/products/HA1139" style="font-weight: bold;text-decoration: underline;">HA1139</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Cerebral cortex
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610392, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Rat<br />Tissue: Cerebral cortex<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA610392" style="font-weight: bold;text-decoration: underline;">HA610392</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488, <a href="/products/HA1139" style="font-weight: bold;text-decoration: underline;">HA1139</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Cerebral cortex
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610392, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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