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Human kappa, AlpHcAbs® Goat antibody Alpaca pacous Monoclonal

Usd: 300

Specification

Bulk Order Quote | Customization Request

Catalog# HA710063

Human kappa, AlpHcAbs® Goat antibody Alpaca pacous Monoclonal

Application

  • WB

  • ICC/IF

  • ELISA

  • IP

  • Flow Cyt

Reactivity
Conjugation

  • unconjugated

Overview

Product Name

Human kappa, AlpHcAbs® Goat antibody Alpaca pacous Monoclonal

Antibody Type

Alpaca Nanobody

Immunogen

Fab region of human IgG

Product Specificity

Human IgG kappa chain

Validated Applications

WB, ICC/IF, ELISA, IP, Flow Cyt

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Storage Buffer

10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Isotype

VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • ELISA

  • 1:5000-1:20000

  • WB

  • 1:5000-1:20000

  • ICC/IF

  • 1:200-1:1000

  • IP

  • 1-2ug/sample

  • Flow Cyt

  • 1µg for 106 cells

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-Human kappa, AlpHcAbs® Goat antibody is designed for detecting human IgG kappa light chain specifically. Anti-Human kappa, AlpHcAbs® Goat antibody is monovalent, recombinant single domain antibody fused to goat IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Human kappa, AlpHcAbs® Goat antibody reacts with human IgG kappa light chain selectively, no reactivity with rabbit, mouse, rat, goat IgG. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .

Background References

暂无

Images

  • Advantages of using an Alpaca Recombinant Secondary<br />1. Smaller size<br />Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.<br />2. Easier to use<br />Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. <br />3. Easier production<br />When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. <br />4. More consistent<br />Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.<br />5. Higher Affinity<br />Nanobodies benefit from consistently high binding affinity values.

    Advantages of using an Alpaca Recombinant Secondary
    1. Smaller size
    Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
    2. Easier to use
    Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
    3. Easier production
    When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
    4. More consistent
    Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
    5. Higher Affinity
    Nanobodies benefit from consistently high binding affinity values.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"