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iFluor™ 594 Conjugated HA tag, AlpHcAbs® Mouse IgG2a Alpaca Monoclonal Antibody

Usd: 500

Specification

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Catalog# HA710216

iFluor™ 594 Conjugated HA tag, AlpHcAbs® Mouse IgG2a Alpaca Monoclonal Antibody

Application

  • WB

  • ICC/IF

  • ELISA

  • FC

Reactivity

  • Species independent

Conjugation

  • iFluor™ 594

Overview

Product Name

iFluor™ 594 Conjugated HA tag, AlpHcAbs® Mouse IgG2a Alpaca Monoclonal Antibody

Antibody Type

Alpaca Nanobody

Immunogen

HA tag fused KLH

Product Specificity

HA tag(YPYDVPDYA)

Species Reactivity

Species independent

Validated Applications

WB, ICC/IF, ELISA, FC

Conjugation

iFluor™ 594

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at –20 ℃ (Avoid freeze / thaw cycles), protect from light

Storage Buffer

Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Isotype

VHH domain of alpaca IgG2b/2c fused to mouse IgG2a Fc(mutation)

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • WB

  • 1:5000-1:20000

  • ICC/IF

  • 1:200-1:1000

  • ELISA

  • 1:5000-1:20000

  • FC

  • 1µg for 106 cells

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor594) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor594) is based on monoclonal, recombinant, mouse IgG2a Fc fused single domain antibody to HA tag coupled to iFluor594. Based on immunofluorescence and/or ELISA, Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor594) detects the HA tag selectively, no reactivity with other proteins.

Background References

暂无

Images

  • Advantages of using an Alpaca Recombinant Secondary<br />1. Smaller size<br />Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.<br />2. Easier to use<br />Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. <br />3. Easier production<br />When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. <br />4. More consistent<br />Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.<br />5. Higher Affinity<br />Nanobodies benefit from consistently high binding affinity values.

    Advantages of using an Alpaca Recombinant Secondary
    1. Smaller size
    Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
    2. Easier to use
    Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
    3. Easier production
    When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
    4. More consistent
    Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
    5. Higher Affinity
    Nanobodies benefit from consistently high binding affinity values.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"