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FAM134B Recombinant Rabbit Monoclonal Antibody [PSH01-94]

Usd: 385

Specification

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Catalog# HA721752

FAM134B Recombinant Rabbit Monoclonal Antibody [PSH01-94]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FAM134B Recombinant Rabbit Monoclonal Antibody [PSH01-94]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FAM134B aa 318-497 / 497.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 55 kDa

Positive Control

HEK-293 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2 cell lysate, TT cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, Jurkat.

Conjugation

unconjugated

Clone Number

PSH01-94

RRID

AB_3072864

Reactivity Data

WB IF-Cell
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Not Recommended
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Endoplasmic reticulum (ER)-anchored autophagy regulator which mediates ER delivery into lysosomes through sequestration into autophagosomes . Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins . Active under basal conditions. Required for collagen quality control in a LIR motif-dependent manner (By similarity). Required for long-term survival of nociceptive and autonomic ganglion neurons.

Background References

1. Kohno S. et al.2019. An N-terminal-truncated isoform of FAM134B (FAM134B-2) regulates starvation-induced hepatic selective ER-phagy. Life Sci Alliance. 17;2(3):e201900340.

2. Mookherjee D. et al. 2021. RETREG1/FAM134B mediated autophagosomal degradation of AMFR/GP78 and OPA1 -a dual organellar turnover mechanism. Autophagy. 17(7):1729-1752.

Subcellular Location

olgi apparatus, cis-Golgi network membrane, Endoplasmic reticulum membrane.

UNIPROT

SwissProt:Q9H6L5 Human

SwissProt:Q8VE91 Mouse

SwissProt:Q5FVM3 Rat

Synonyms

Reticulophagy regulator 1

RETREG1

Reticulophagy receptor 1

FAM134B

JK1

Images

  • Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (<a href="/products/HA721752" style="font-weight: bold;text-decoration: underline;">HA721752</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (30 µg/Lane)<br />Lane 2: U-2 OS cell lysate (30 µg/Lane)<br />Lane 3: MCF7 cell lysate (30 µg/Lane)<br />Lane 4: HeLa cell lysate (30 µg/Lane)<br />Lane 5: HepG2 cell lysate (30 µg/Lane)<br />Lane 6: TT cell lysate (30 µg/Lane)<br />Lane 7: Jurkat cell lysate (30 µg/Lane)<br />Lane 8: NIH/3T3 cell lysate (30 µg/Lane)<br />Lane 9: C2C12 cell lysate (30 µg/Lane)<br />Lane 10: PC-12 cell lysate (30 µg/Lane)<br />Lane 11: C6 cell lysate (30 µg/Lane)<br />Lane 12: Mouse liver tissue lysate (30 µg/Lane)<br />Lane 13: Rat liver tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 55 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 6 minutes 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721752" style="font-weight: bold;text-decoration: underline;">HA721752</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (HA721752) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate (30 µg/Lane)
    Lane 2: U-2 OS cell lysate (30 µg/Lane)
    Lane 3: MCF7 cell lysate (30 µg/Lane)
    Lane 4: HeLa cell lysate (30 µg/Lane)
    Lane 5: HepG2 cell lysate (30 µg/Lane)
    Lane 6: TT cell lysate (30 µg/Lane)
    Lane 7: Jurkat cell lysate (30 µg/Lane)
    Lane 8: NIH/3T3 cell lysate (30 µg/Lane)
    Lane 9: C2C12 cell lysate (30 µg/Lane)
    Lane 10: PC-12 cell lysate (30 µg/Lane)
    Lane 11: C6 cell lysate (30 µg/Lane)
    Lane 12: Mouse liver tissue lysate (30 µg/Lane)
    Lane 13: Rat liver tissue lysate (30 µg/Lane)

    Predicted band size: 55 kDa
    Observed band size: 70 kDa

    Exposure time: 6 minutes 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Jurkat cells labeling FAM134B with Rabbit anti-FAM134B antibody (<a href="/products/HA721752" style="font-weight: bold;text-decoration: underline;">HA721752</a>) at 1/100 dilution.<br /><br />Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM134B antibody (<a href="/products/HA721752" style="font-weight: bold;text-decoration: underline;">HA721752</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Jurkat cells labeling FAM134B with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution.

    Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation