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SMCC Recombinant Rabbit Monoclonal Antibody [PSH06-06]

Usd: 385

Specification

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Catalog# HA722505

SMCC Recombinant Rabbit Monoclonal Antibody [PSH06-06]

Application

  • ELISA

Reactivity

  • Species independent

Conjugation

  • unconjugated

Overview

Product Name

SMCC Recombinant Rabbit Monoclonal Antibody [PSH06-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Fusion protein With SMCC.

Species Reactivity

Species independent

Validated Applications

ELISA

Conjugation

unconjugated

Clone Number

PSH06-06

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA

  • 1:20,000

Target

Function

Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a heterobifunctional amine-to-sulfhydryl crosslinker, which contains two reactive groups at opposite ends: N-hydroxysuccinimide-ester and maleimide, reactive with amines and thiols respectively. SMCC is often used in bioconjugation to link proteins with other functional entities (fluorescent dyes, tracers, nanoparticles, cytotoxic agents). For example, a targeted anticancer agent – trastuzumab emtansine (antibody-drug conjugate containing an antibody trastuzumab chemically linked to a highly potent drug DM-1) – is prepared using SMCC reagent. In a pharmacokinetics study, the CX-DM1-containing ADCs exhibited stability comparable to that of SMCC-DM1-containing ADCs. SMCC is a classic noncleavable linker that has been employed in Kadcyla. However, SMCC-based conjugates are still limited due to their instability in the circulatory system and hydrophobic properties.

Background References

1. Lambert J.M., Chari R.V. Ado-trastuzumab emtansine (T-DM1): an antibody-drug conjugate (ADC) for HER2− positive breast cancer. J Med Chem. 2014;57:6949–6964

2. Dovgan I., Kolodych S., Koniev O., Wagner A. 2-(Maleimidomethyl)-1,3-dioxanes (MD): a serum-stable self-hydrolysable hydrophilic alternative to classical maleimide conjugation. Sci Rep. 2016;6:30835

Synonyms

SMCC antibody

Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate antibody

Images

  • Indirect ELISA analysis of SMCC was performed by coating wells of a 96-well plate with 50 µL per well of different SMCC conjugations diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of SMCC monoclonal antibody serial diluted starting from a concentration of 1µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Indirect ELISA analysis of SMCC was performed by coating wells of a 96-well plate with 50 µL per well of different SMCC conjugations diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of SMCC monoclonal antibody serial diluted starting from a concentration of 1µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"