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Estriol Recombinant Rabbit Monoclonal Antibody [PSH15-86]

Usd: 385

Specification

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Catalog# HA723795

Estriol Recombinant Rabbit Monoclonal Antibody [PSH15-86]

Application

  • ELISA

Reactivity

  • Species independent

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Estriol Recombinant Rabbit Monoclonal Antibody [PSH15-86]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Species independent

Validated Applications

ELISA

Conjugation

unconjugated

Clone Number

PSH15-86

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Estriol (E3), also spelled oestriol, is a steroid, a weak estrogen, and a minor female sex hormone. It is one of three major endogenous estrogens, the others being estradiol and estrone. Levels of estriol in women who are not pregnant are almost undetectable. However, during pregnancy, estriol is synthesized in very high quantities by the placenta and is the most produced estrogen in the body by far, although circulating levels of estriol are similar to those of other estrogens due to a relatively high rate of metabolism and excretion. Relative to estradiol, both estriol and estrone have far weaker activity as estrogens. In addition to its role as a natural hormone, estriol is used as a medication, for instance in menopausal hormone therapy; for information on estriol as a medication, see the estriol (medication) article.

Background References

1. Long J et al. An estriol-eluting pessary to treat pelvic organ prolapse. Sci Rep. 2022 Nov

2. Zhu L et al. Estriol dissolving microneedle patches for protection against ionizing radiation-induced injury. Eur J Pharm Sci. 2021 Aug

Subcellular Location

Secreted.

Synonyms

E3 antibody

Images

  • Indirect ELISA analysis of Estriol was performed by coating wells of a 96-well plate with 50 µl per well of Estriol-BSA antigen diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of an Estriol antibody starting at a concentration of 4 µg/mL and serially diluting it to a concentration of 3.9 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Indirect ELISA analysis of Estriol was performed by coating wells of a 96-well plate with 50 µl per well of Estriol-BSA antigen diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of an Estriol antibody starting at a concentration of 4 µg/mL and serially diluting it to a concentration of 3.9 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Competitive ELISA analysis of Estriol / Estradiol / Estrone / 16-Epiestriol was performed by coating wells of a 96-well plate with 50 µl per well of Estriol-BSA antigen diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. The wells of the plate were washed, blocked with StartingBlock blocking buffer, and then incubated with 50 µL per well of an Estriol antibody at a concentration of 4 µg/mL along with Estriol / Estradiol / Estrone / 16-Epiestriol / PBS buffer only for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Competitive ELISA analysis of Estriol / Estradiol / Estrone / 16-Epiestriol was performed by coating wells of a 96-well plate with 50 µl per well of Estriol-BSA antigen diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. The wells of the plate were washed, blocked with StartingBlock blocking buffer, and then incubated with 50 µL per well of an Estriol antibody at a concentration of 4 µg/mL along with Estriol / Estradiol / Estrone / 16-Epiestriol / PBS buffer only for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"