iFluor™ 594 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]
Catalog# HA723821F
iFluor™ 594 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]
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IHC-Fr
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Mouse
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iFluor™ 594
Overview
Product Name
iFluor™ 594 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within N-terminal human Iba1.
Species Reactivity
Mouse
Validated Applications
IHC-Fr
Molecular Weight
Predicted band size: 17 kDa
Conjugation
iFluor™ 594
Clone Number
JM36-62
Reactivity Data
| IHC-Fr | |
|---|---|
| Mouse |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:100
Target
Function
Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).
Background References
1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).
2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).
Subcellular Location
Cytoskeleton, ruffle membrane, phagocytic cup.
UNIPROT
Synonyms
AIF 1 antibody
AIF-1 antibody
Aif1 antibody
AIF1 protein antibody
AIF1_HUMAN antibody
Allograft inflammatory factor 1 antibody
Allograft inflammatory factor 1 splice variant G antibody
allograft inflammatory factor-1 splice variant Hara-1 antibody
balloon angioplasty responsive transcription antibody
BART 1 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/100
Antigen retrieval: Not required
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"