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BARD1 Recombinant Rabbit Monoclonal Antibody [PSH22-30]

Usd: 385

Specification

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Catalog# HA724291

BARD1 Recombinant Rabbit Monoclonal Antibody [PSH22-30]

Application

  • WB

  • IP

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

BARD1 Recombinant Rabbit Monoclonal Antibody [PSH22-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human BARD1 aa 1-450.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 87 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HeLa treated with 2μM MG-132 for 18 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH22-30

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. Most, if not all, BRCA1 heterodimerizes with BARD1 in vivo. BARD1 and BRCA1 form a heterodimer via their N-terminal RING finger domains. The BARD1-BRCA1 interaction is observed in vivo and in vitro and is essential for BRCA1 stability. BARD1 shares homology with the two most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes, and developmentally important genes such as the polycomb group of genes. The BARD1 protein also contains three tandem ankyrin repeats.

Background References

1. Witus SR et al. BRCA1/BARD1 is a nucleosome reader and writer. Trends Biochem Sci. 2022 Jul

2. Hawsawi YM et al. BARD1 mystery: tumor suppressors are cancer susceptibility genes. BMC Cancer. 2022 Jun

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q99728 Human

Synonyms

BARD-1 antibody

Bard1 antibody

BARD1_HUMAN antibody

BRCA1 associated RING domain 1 antibody

BRCA1 associated RING domain gene 1 antibody

BRCA1 associated RING domain protein 1 antibody

BRCA1-associated RING domain protein 1 antibody

Images

  • Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: HEK-293 cell lysate<br />Lane 4: MDA-MB-231 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 87/100 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (HA724291) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HEK-293 cell lysate
    Lane 4: MDA-MB-231 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 87/100 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724291) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 2μM MG-132 for 18 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 87/100 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (HA724291) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 2μM MG-132 for 18 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 87/100 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724291) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • BARD1 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    BARD1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA724291 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724291 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA724291 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA724291 in HeLa cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 3 minutes; ECL: K1801

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