Biotin Conjugated Mouse Apolipoprotein E Recombinant Rabbit Monoclonal Antibody [PSH16-84] - Detector
Overview
Product Name
Biotin Conjugated Mouse Apolipoprotein E Recombinant Rabbit Monoclonal Antibody [PSH16-84] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Apolipoprotein E aa 19-311 (HA211210).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse Apolipoprotein E protein (HA211210).
Conjugation
Biotin
Clone Number
PSH16-84
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-83] to Mouse Apolipoprotein E antibody (Capture) (HA725221) and Recombinant Mouse Apolipoprotein E protein (HA211210) as the standard. The reference range value is 3.125-200 ng/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Apolipoprotein-E (apoE) is a protein component of plasma lipoproteins that mediates the binding, internalization and catabolism of lipoprotein particles. It can serve as a ligand for several lipoprotein receptors, including the LDL (ApoB/E) receptor and the hepatic apoE (chylomicron remnant) receptor. apoE is produced in most organs and occurs in all plasma lipoprotein fractions, constituting 10-20% of VLDL (very low density lipoprotein) and 1-2% of HDL (high density lipoprotein). Three major isoforms of apoE have been described in human (E2, E3 and E4) which differ by only one or two amino acids. Estrogen receptor has been shown to upregulate apoE gene expression via the ERa-mediated pathway, indicating a potential role for apoE in atherosclerosis. This is consistent with studies in mice in which plasma apoE levels were raised, thereby protecting the mice from diet-induced atherosclerosis. apoE has also been shown to be a potent inhibitor of proliferation and thus may play a role in angiogenesis, tumor cell growth and metastasis.
Background References
1. Yang LG et al. Apolipoprotein E in lipid metabolism and neurodegenerative disease. Trends Endocrinol Metab. 2023 Aug
2. Chen Y et al. Apolipoprotein E: Structural Insights and Links to Alzheimer Disease Pathogenesis. Neuron. 2021 Jan
Subcellular Location
Secreted, extracellular space, extracellular matrix, Extracellular vesicle, Endosome, multivesicular body.
UNIPROT
Synonyms
AD2 antibody
Apo-E antibody
APOE antibody
APOEA antibody
Apolipoprotein E antibody
Apolipoprotein E3 antibody
ApolipoproteinE antibody
Apoprotein antibody
LDLCQ5 antibody
LPG antibody
Images
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![Sandwich ELISA analysis of Mouse Apolipoprotein E matched pair antibodies<br /><br />Capture: <a href="/products/HA725221" style="font-weight: bold;text-decoration: underline;">HA725221</a>, Mouse Apolipoprotein E Rabbit mAb [PSH16-83]<br />Detector: <a href="/products/HA725222" style="font-weight: bold;text-decoration: underline;">HA725222</a>, Mouse Apolipoprotein E Rabbit mAb [PSH16-84]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725221" style="font-weight: bold;text-decoration: underline;">HA725221</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein E protein (<a href="/products/HA211210" style="font-weight: bold;text-decoration: underline;">HA211210</a>) starting from 200,000 pg/ml to 2.0 pg/ml and detect antibody (<a href="/products/HA725222" style="font-weight: bold;text-decoration: underline;">HA725222</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725222B_1.png?v=20260604174734)
Sandwich ELISA analysis of Mouse Apolipoprotein E matched pair antibodies
Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83]
Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725221) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein E protein (HA211210) starting from 200,000 pg/ml to 2.0 pg/ml and detect antibody (HA725222, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native APOE in mouse liver, C2C12 extract samples based on a 1mg/ml extract load.<br /><br />Capture: <a href="/products/HA725221" style="font-weight: bold;text-decoration: underline;">HA725221</a>, Mouse Apolipoprotein E Rabbit mAb [PSH16-83]<br />Detector: <a href="/products/HA725222" style="font-weight: bold;text-decoration: underline;">HA725222</a>, Mouse Apolipoprotein E Rabbit mAb [PSH16-84]<br /><br />The concentrations of APOE were measured in duplicates, interpolated from the APOE standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOE concentration was determined to be 813 ng/ml in mouse liver extract, There was no detected signal in C2C12 cell extract.](https://storage.huabio.cn/huabio/productImg/HA725222B_2.png?v=20260604174734)
Interpolated concentrations of native APOE in mouse liver, C2C12 extract samples based on a 1mg/ml extract load.
Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83]
Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84]
The concentrations of APOE were measured in duplicates, interpolated from the APOE standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOE concentration was determined to be 813 ng/ml in mouse liver extract, There was no detected signal in C2C12 cell extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"