Collagen XVII Recombinant Rabbit Monoclonal Antibody [SR46-05] - BSA and Azide free
Catalog# HA750030
Collagen XVII Recombinant Rabbit Monoclonal Antibody [SR46-05] - BSA and Azide free
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WB
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Collagen XVII Recombinant Rabbit Monoclonal Antibody [SR46-05] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Collagen XVII aa 1313-1420 / 1497.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Tissue, IHC-P, IP
Target Molecular Weight
Predicted band size: 148 kDa
Positive Control
A431 cell lysate, mouse skin tissue lysate, rat skin tissue lysate, HUVEC, human skin tissue, mouse skin tissue, rat skin tissue.
Conjugation
unconjugated
Clone Number
SR46-05
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Tissue
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1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
Collagen XVII, previously called BP180, is a transmembrane protein which plays a critical role in maintaining the linkage between the intracellular and the extracellular structural elements involved in epidermal adhesion. COL17A1 is the official name of the gene. It encodes the alpha chain of type XVII collagen. Collagen XVII is a transmembrane protein, like collagen XIII, XXIII and XXV. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. It also appears to be a key protein in maintaining the integrity of the corneal epithelium.[5] Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa,[6] as well as recurrent corneal erosions, and expression of this gene is abnormal in various cancers. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form.
Background References
1. Jonsson F., Bystroem B., Davidson A.E., et al. Mutations in collagen, type XVII, alpha 1 (COL17A1) cause epithelial recurrent erosion dystrophy (ERED). Hum. Mutat. 36:463-473(2015).
2. Burkard T.R., Planyavsky M., Kaupe I., et al. Initial characterization of the human central proteome. BMC Syst. Biol. 5:17-17(2011).
Tissue Specificity
Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
Post-translational Modification
The intracellular/endo domain is disulfide-linked.; Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.; The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544.
Subcellular Location
Cell junction, Membrane, Secreted
Synonyms
180 kDa bullous pemphigoid antigen 2 antibody
Alpha 1 type XVII collagen antibody
BA16H23.2 antibody
BP 180 antibody
BP180 antibody
BPA 2 antibody
BPAG 2 antibody
BPAG2 antibody
Bullous pemphigoid antigen 2 antibody
COL17A1 antibody
ExpandImages
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Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (HA750030) at 1/5,000 dilution.
Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: Mouse skin tissue lysate (20 µg/Lane)
Lane 3: Rat skin tissue lysate (20 µg/Lane)
Predicted band size: 148 kDa
Observed band size: 130、180 kDa
Exposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750030) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (HA750030) at 1/1,000 dilution.
Lane 1: Wild-type caski whole cell lysate (10 µg).
Lane 2/3: Collagen XVII knockdown caski whole cell lysate (10 µg).
Predicted band size: 148 kDa
Observed band size: 130、180 kDa
Exposure time: 1 minutes 40 seconds; ECL: K1801;4-20% SDS-PAGE gel.
HA750030 was shown to specifically react with Collagen XVII in wild-type caski cells. Weakened bands were observed when Collagen XVII knockdown samples were tested. Wild-type and Collagen XVII knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA750030) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen XVII antibody (HA750030) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody (HA750030) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Collagen XVII antibody (HA750030) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Mouse
Site: skin
Sample: Paraffin-embedded section
Antibody concentration: 1/200
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"