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DUSP6 Recombinant Rabbit Monoclonal Antibody [SR39-09] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750032

DUSP6 Recombinant Rabbit Monoclonal Antibody [SR39-09] - BSA and Azide free

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Chicken

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

DUSP6 Recombinant Rabbit Monoclonal Antibody [SR39-09] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DUSP6 aa 325-381 / 381.

Species Reactivity

Human, Mouse, Rat (Predicted: Chicken)

Predicted species support after-sales service

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 42 kDa

Positive Control

HepG2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Human lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human pancreas tissue, mouse pancreas tissue, human gastric carcinoma tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

SR39-09

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Tissue

  • 1:50

  • IHC-P

  • 1:200-1:1,000

Target

Function

Dual specificity phosphatase 6 (DUSP6) is an enzyme that in humans is encoded by the DUSP6 gene. The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas and, unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. Upregulation of MKP-3 has been shown to alleviate chronic postoperative pain.

Background References

1. Bertin S et al. Dual-specificity phosphatase 6 regulates CD4(+) T-cell functions and restrains spontaneous colitis in IL-10-deficient mice. Mucosal Immunol N/A:N/A (2014).

2. Wales S et al. Global MEF2 target gene analysis in cardiac and skeletal muscle reveals novel regulation of DUSP6 by p38MAPK-MEF2 signaling. Nucleic Acids Res 42:11349-62 (2014).

Sequence Similarity

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Tissue Specificity

Expressed in keratinocytes (at protein level).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q16828 Human

SwissProt: Q9DBB1 Mouse

SwissProt: Q64346 Rat

Synonyms

Dual specificity phosphatase 6 antibody

Dual specificity phosphatase 6 isoform a antibody

Dual specificity protein phosphatase 6 antibody

Dual specificity protein phosphatase PYST1 antibody

DUS6_HUMAN antibody

DUSP 6 antibody

DUSP 6a antibody

Dusp6 antibody

DUSP6a antibody

HH19 antibody

Expand

Images

  • Western blot analysis of DUSP6 on different lysates with Rabbit anti-DUSP6 antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: RAW264.7 cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: Human lung tissue lysate<br />Lane 5: Mouse brain tissue lysate<br />Lane 6: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DUSP6 on different lysates with Rabbit anti-DUSP6 antibody (HA750032) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: RAW264.7 cell lysate
    Lane 3: C6 cell lysate
    Lane 4: Human lung tissue lysate
    Lane 5: Mouse brain tissue lysate
    Lane 6: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-DUSP6 antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-DUSP6 antibody (HA750032) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750032) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-DUSP6 antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-DUSP6 antibody (HA750032) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750032) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DUSP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DUSP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750032, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DUSP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750032" style="font-weight: bold;text-decoration: underline;">HA750032</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DUSP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750032, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

DUSP6 Recombinant Rabbit Monoclonal Antibody [SR39-09]

Application: WB,IF-Tissue,IHC-P

Reactivity: Human,Mouse,Rat,Chicken

Conjugate: unconjugated