Phospho-Smad5 (S463 + S465) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SY09-03] (HA750089)
Catalog# HA750089
Rabbit Monoclonal to Phospho-Smad5 (S463/465)
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Smad5 (S463 + S465) on different lysates with Rabbit anti-Phospho-Smad5 (S463 + S465) antibody (HA750089) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 20 ng/mL TGFβ1 for 15 minutes whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750089) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Smad5 (S463 + S465) on different lysates with Rabbit anti-Phospho-Smad5 (S463 + S465) antibody (HA750089) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 20 ng/mL TGFβ1 for 15 minutes whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750089) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.