Calponin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SI67-01] (HA750094)
Catalog# HA750094
Rabbit Monoclonal to Calponin
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Calponin on different lysates with Rabbit anti-Calponin antibody (HA750094) at 1/1,000 dilution.
Lane 1: Mouse stomach tissue lysate
Lane 2: Rat stomach tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 35 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750094) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse stomach tissue lysate
Lane 2: Rat stomach tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 35 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750094) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.