ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19] - BSA and Azide free
Catalog# HA750181
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19] - BSA and Azide free
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WB
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ROCK1 aa aa 1104-1149 / 1,354.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Tissue, IHC-P, IP
Target Molecular Weight
Predicted band size: 158 kDa
Positive Control
A431 cell lysate, Human lung tissue lysate, Mouse spleen tissue lysate, C6 cell lysate, L6 cell lysate, human thyroid tissue, mouse thyroid tissue, rat thyroid tissue.
Conjugation
unconjugated
Clone Number
ST05-19
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Rho, the Ras-related small GTPase, is responsible for the regulation of Actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka, for Rho-associated coil-containing protein kinase). Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase.
Background References
1. Gentry EG et al. Rho Kinase Inhibition as a Therapeutic for Progressive Supranuclear Palsy and Corticobasal Degeneration. J Neurosci 36:1316-23 (2016).
2. Cai SD et al. MicroRNA-144 inhibits migration and proliferation in rectal cancer by downregulating ROCK-1. Mol Med Rep 12:7396-402 (2015).
Sequence Similarity
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Tissue Specificity
Detected in blood platelets.
Post-translational Modification
Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Subcellular Location
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane.
Synonyms
coiled-coil-containing protein kinase 1 antibody
coiled-coil-containing protein kinase I antibody
MGC131603 antibody
MGC43611 antibody
p160 Rhoassociated coiled coil-forming protein kinase antibody
p160 ROCK-1 antibody
p160 ROCK1 antibody
p160ROCK antibody
PRO0435 antibody
Renal carcinoma antigen NY REN 35 antibody
ExpandImages
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This data was developed using ET1609-53, the same antibody clone in a different buffer formulation.
Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ET1609-53) at 1/1,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: Human lung tissue lysate (20 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: L6 cell lysate (20 µg/Lane)
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1609-53, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-ROCK1 antibody (ET1609-53) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1609-53, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue with Rabbit anti-ROCK1 antibody (ET1609-53) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1609-53, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat thyroid tissue with Rabbit anti-ROCK1 antibody (ET1609-53) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19]
Application: WB,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
