Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [ST50-05] (HA750185)
Catalog# HA750185
Rabbit Monoclonal to Cytokeratin 7
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
☑ Knockdown (KD)
All lanes: Western blot analysis of Cytokeratin 7 with anti-Cytokeratin 7 antibody [ST50-05] (HA750185) at 1:1,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate (20 µg).
Lane 3/4: Cytokeratin 7 fragment 1 knockdown Hela whole cell lysate (20 µg).
Lane 5/6: Cytokeratin 7 fragment 2 knockdown Hela whole cell lysate (20 µg).
ET1609-62 was shown to specifically react with Cytokeratin 7 in wild-type Hela cells. Weakened bands were observed when Cytokeratin 7 knockdown samples were tested. Wild-type and Cytokeratin 7 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-62, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of Cytokeratin 7 with anti-Cytokeratin 7 antibody [ST50-05] (HA750185) at 1:1,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate (20 µg).
Lane 3/4: Cytokeratin 7 fragment 1 knockdown Hela whole cell lysate (20 µg).
Lane 5/6: Cytokeratin 7 fragment 2 knockdown Hela whole cell lysate (20 µg).
ET1609-62 was shown to specifically react with Cytokeratin 7 in wild-type Hela cells. Weakened bands were observed when Cytokeratin 7 knockdown samples were tested. Wild-type and Cytokeratin 7 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-62, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.