BCL2A1 Recombinant Rabbit Monoclonal Antibody [SC05-42] - BSA and Azide free
Catalog# HA750205
BCL2A1 Recombinant Rabbit Monoclonal Antibody [SC05-42] - BSA and Azide free
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WB
-
IHC-P
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Human
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Mouse
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ET1610-20
含抗保成分
-
unconjugated
Overview
Product Name
BCL2A1 Recombinant Rabbit Monoclonal Antibody [SC05-42] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human BCL2A1 aa 2-48 / 175.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 20 kDa
Positive Control
Human tonsil tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, 293, human liver tissue, human kidney tissue lysates.
Conjugation
unconjugated
Clone Number
SC05-42
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:50-1:400
Target
Function
The Bcl-2 family of proteins is characterized by its ability to modulate cell death under a broad range of physiological conditions. Bcl-2 and Bcl-xL function to inhibit apoptosis while other members of the Bcl-2 family, Bax, Bad, Bak and Bcl-xS, oppose death-suppressing effects. An additional member of the family, A1 (also designated Bfl-1), dimerizes with both Bcl-2 and Bax and has been identified as a hematopoietic- specific, early inducible gene. While A1 demonstrates life promoting properties similar to those of Bcl-2, its function may be more temporally regulated during myeloid differentiation and dependent on additional growth stimuli to confer its life promoting properties. A1 is abundantly expressed in bone marrow and at low levels in other tissues. There is evidence that a correlation exists between a high expression of the A1 gene product and stomach cancer.
Background References
1. Jauharoh SN et al. SS-A/Ro52 promotes apoptosis by regulating Bcl-2 production. Biochem Biophys Res Commun 417:582-7 (2012).
2. Fardin P et al. Induction of epithelial mesenchimal transition and vasculogenesis in the lenses of Dbl oncogene transgenic mice. PLoS One 4:e7058 (2009).
Sequence Similarity
Belongs to the Bcl-2 family.
Tissue Specificity
Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.
Subcellular Location
Cytoplasm.
Synonyms
ACC 1 antibody
ACC 2 antibody
B2LA1_HUMAN antibody
Bcl 2 related protein A1 antibody
Bcl-2-like protein 5 antibody
Bcl-2-related protein A1 antibody
BCL2 related protein A1 antibody
Bcl2-L-5 antibody
BCL2A1 antibody
BCL2L5 antibody
ExpandImages
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Western blot analysis of BCL2A1 on human kidney tissue lysates with Rabbit anti-BCL2A1 antibody (HA750205) at 1/500 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 26 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750205) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-BCL2A1 antibody (HA750205) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750205) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BCL2A1 antibody (HA750205) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750205) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-BCL2A1 antibody (HA750205) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750205) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BCL2A1 antibody (HA750205) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750205) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BCL2A1 antibody (HA750205) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750205) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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