Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08] - BSA and Azide free
Catalog# HA750208
Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08] - BSA and Azide free
-
WB
-
IHC-P
-
IP
-
IF-Cell
-
Human
-
unconjugated
Overview
Product Name
Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Aurora B aa 1-50 / 344.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP, IF-Cell
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
Hela cell lysate, MCF-7 cell lysate, human tonsil tissue, HeLa.
Conjugation
unconjugated
Clone Number
SC55-08
Reactivity Data
| WB | IHC-P | IP | IF-Cell | |
|---|---|---|---|---|
| Human |
|
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/ threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1), and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.
Background References
1. Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016).
2. Jiang J et al. Cardiac myosin binding protein C regulates postnatal myocyte cytokinesis. Proc Natl Acad Sci U S A 112:9046-51 (2015).
Sequence Similarity
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Tissue Specificity
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Post-translational Modification
The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.; Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Subcellular Location
Nucleus, Cytoplasm, Chromosome.
Synonyms
AIK2 antibody
AIM-1 antibody
AIM1 antibody
ARK-2 antibody
ARK2 antibody
AurB antibody
AURKB antibody
AURKB_HUMAN antibody
Aurora 1 antibody
Aurora and Ipl1 like midbody associated protein 1 antibody
ExpandImages
-
This data was developed using ET1610-25, the same antibody clone in a different buffer formulation.
Western blot analysis of Aurora B on different lysates with Rabbit anti-Aurora B antibody (ET1610-25) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: 293T (Human embryonic kidney cell) cell lysate
Lane 3: SW620 (Human colon cancer cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1610-25, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 39 kDa
Observed band size: 39 kDa -
☑ Knockdown (KD)
This data was developed using ET1610-25, the same antibody clone in a different buffer formulation.
All lanes: Western blot analysis of Aurora B with anti-Aurora B antibody (ET1610-25) at 1:500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2: Aurora B knockdown Hela whole cell lysate (10 µg).
ET1610-25 was shown to specifically react with Aurora B in wild-type Hela cells. Weakened band was observed when Aurora B knockdown sample was tested. Wild-type and Aurora B knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-25, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1610-25, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Aurora B antibody. Counter stained with hematoxylin. -
This data was developed using ET1610-25, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa cells labeling Aurora B with Rabbit anti-Aurora B antibody (ET1610-25) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aurora B antibody (ET1610-25) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
This data was developed using ET1610-25, the same antibody clone in a different buffer formulation.
Aurora B was immunoprecipitated from 0.2 mg HepG2 cell lysate with ET1610-25 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1610-25 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (input)
Lane 2: ET1610-25 IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of ET1610-25 in HepG2 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 20 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"