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S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750209

S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02] - BSA and Azide free

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IHC-Fr

  • IF-Cell

  • FC

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

With BSA and Azide
Predicted reactivity

  • Goat

  • Cynomolgus monkey

  • Green monkey

  • Pig

  • Rabbit

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal human S100 beta.

Species Reactivity

Human, Mouse, Rat, Zebrafish (Predicted: Goat, Cynomolgus monkey, Green monkey, Pig, Rabbit)

Predicted species support after-sales service

Validated Applications

WB, IF-Tissue, IHC-P, IHC-Fr, IF-Cell, FC, IP

Molecular Weight

Predicted band size: 11 kDa

Positive Control

Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, mouse liver tissue lysates, SK-MEL-28 cell lysate, Rat brain tissue lysate, zebrafish tissue lysates, A375.

Conjugation

unconjugated

Clone Number

SC57-02

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:500-1:1,000

  • IHC-P

  • 1:2,000

  • IHC-Fr

  • 1:1,000

  • IF-Cell

  • 1:200

  • FC

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

S100 calcium-binding protein B (S100B) is a protein of the S100 protein family. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker.

Background References

1. Duan K et al. S100-beta aggravates spinal cord injury via activation of M1 macrophage phenotype. J Musculoskelet Neuronal Interact. 2021 Sep

2. Hanin A et al. Neuron Specific Enolase, S100-beta protein and progranulin as diagnostic biomarkers of status epilepticus. J Neurol. 2022 Jul

Sequence Similarity

Belongs to the S-100 family.

Tissue Specificity

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Subcellular Location

Cytoplasm, Nucleus, Secreted.

UNIPROT

SwissProt: P04271 Human

SwissProt: P50114 Mouse

SwissProt: P04631 Rat

Synonyms

NEF antibody

Protein S100 B antibody

Protein S100-B antibody

S 100 calcium binding protein beta chain antibody

S 100 protein beta chain antibody

S-100 protein beta chain antibody

S-100 protein subunit beta antibody

S100 antibody

S100 calcium binding protein beta (neural) antibody

S100 calcium-binding protein B antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1:1,000

    Antigen retrieval: Not required

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:500

    Application: IF-tissue

    Species: Mouse

    Site: Hippocampus

    Sample: Paraffin-embedded section

    Antibody concentration: 1:500

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-S100 beta antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-S100 beta antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-S100 beta antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-S100 beta antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-S100 beta antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-S100 beta antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of S100 beta on mouse liver tissue lysates with Rabbit anti-S100 beta antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 11 kDa<br />Observed band size: 11 kDa<br /><br />Exposure time: 2 minutes;<br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of S100 beta on mouse liver tissue lysates with Rabbit anti-S100 beta antibody (HA750209) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa

    Exposure time: 2 minutes;
    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750209) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of S100 beta on different lysates with Rabbit anti-S100 beta antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution.<br /><br />Lane 1: SK-MEL-28 cell lysate (15 µg/Lane)<br />Lane 2: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 11 kDa<br />Observed band size: 11 kDa<br /><br />Exposure time: 3 minutes 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of S100 beta on different lysates with Rabbit anti-S100 beta antibody (HA750209) at 1/2,000 dilution.

    Lane 1: SK-MEL-28 cell lysate (15 µg/Lane)
    Lane 2: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 11 kDa
    Observed band size: 11 kDa

    Exposure time: 3 minutes 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><br />Predicted band size: 11 kDa<br />Observed band size: 14 kDa

    Western blot analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750209, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa

  • Immunocytochemistry analysis of A375 cells labeling S100 beta with Rabbit anti-S100 beta antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/200 dilution.<br /><br />Cells were fixed in ice-cold 100% methanol for 5 minutes, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100 beta antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of A375 cells labeling S100 beta with Rabbit anti-S100 beta antibody (HA750209) at 1/200 dilution.

    Cells were fixed in ice-cold 100% methanol for 5 minutes, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100 beta antibody (HA750209) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of A375 cells labeling S100 beta.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA750209" style="font-weight: bold;text-decoration: underline;">HA750209</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A375 cells labeling S100 beta.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA750209, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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