Skip to content

Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA750214

Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human TPH1 aa 372-419 / 444.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Molecular Weight

Predicted band size: 51 kDa

Positive Control

MDA-MB-231 cell lysate, LNCaP cell lysate, HL-60 cell lysate, THP-1 cell lysate, MCF7 cell lysate, human brain tissue, rat brain tissue, mouse cerebellum tissue, Hela.

Conjugation

unconjugated

Clone Number

SC53-07

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland. Alteration of TPH function may be a key factor in the pathology of several neuropsychiatric disorders associated with serotonin, including depression, aggression, alcoholism and schizophrenia. For instance, L-DOPA, which is used as a common therapy for Parkinson’s disease (PD) patients, inhibits TPH function, which subsequently, is thought to contribute to the onset of depression in PD patients.

Background References

1. Cheng HH et al. Quiescent and proliferative fibroblasts exhibit differential p300 HAT activation through control of 5-methoxytryptophan production. PLoS One 9:e88507 (2014).

2. Dempsie Y et al. Dexfenfluramine and the oestrogen-metabolizing enzyme CYP1B1 in the development of pulmonary arterial hypertension. Cardiovasc Res 99:24-34 (2013).

Sequence Similarity

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Tissue Specificity

Isoform 2 seems to be less widely expressed than isoform 1.

Subcellular Location

Cytosol, neuron projection.

UNIPROT

SwissProt: P17752 Human

SwissProt: P17532 Mouse

SwissProt: P09810 Rat

Synonyms

Indoleacetic acid 5 hydroxylase antibody

L tryptophan hydroxylase antibody

MGC119994 antibody

TPH 1 antibody

TPH antibody

TPH1 antibody

TPH1_HUMAN antibody

TPRH antibody

TRPH antibody

Tryptophan 5 hydroxylase 1 antibody

Expand

Images

  • Western blot analysis of Tryptophan Hydroxylase 1 (TPH1) on different lysates with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>) at 1/1,000 dilution.<br /><br />Lane 1: MDA-MB-231 cell lysate<br />Lane 2: LNCaP cell lysate<br />Lane 3: HL-60 cell lysate<br />Lane 4: THP-1 cell lysate<br />Lane 5: MCF7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 72 kDa<br /><br />Exposure time: 3 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Tryptophan Hydroxylase 1 (TPH1) on different lysates with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/1,000 dilution.

    Lane 1: MDA-MB-231 cell lysate
    Lane 2: LNCaP cell lysate
    Lane 3: HL-60 cell lysate
    Lane 4: THP-1 cell lysate
    Lane 5: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 72 kDa

    Exposure time: 3 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750214) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>) at 1/1,000 dilution.<br /><br />Lane 1: MDA-MB-231-si NT cell lysate (10 µg/Lane)<br />Lane 2: MDA-MB-231-si TPH1 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 51 kDa<br />Observed band size: 72 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br /><a href="/products/ET1610-37" style="font-weight: bold;text-decoration: underline;">ET1610-37</a> was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-37" style="font-weight: bold;text-decoration: underline;">ET1610-37</a>, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody (HA750214) at 1/1,000 dilution.

    Lane 1: MDA-MB-231-si NT cell lysate (10 µg/Lane)
    Lane 2: MDA-MB-231-si TPH1 cell lysate (10 µg/Lane)

    Predicted band size: 51 kDa
    Observed band size: 72 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    ET1610-37 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-37, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Tryptophan Hydroxylase 1 (TPH1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Tryptophan Hydroxylase 1 (TPH1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Tryptophan Hydroxylase 1 (TPH1) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA750214" style="font-weight: bold;text-decoration: underline;">HA750214</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Tryptophan Hydroxylase 1 (TPH1) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750214, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"