Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free
Catalog# HA750214
Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
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unconjugated
Overview
Product Name
Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TPH1 aa 372-419 / 444.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
MDA-MB-231 cell lysate, LNCaP cell lysate, HL-60 cell lysate, THP-1 cell lysate, MCF7 cell lysate, human brain tissue, rat brain tissue, mouse cerebellum tissue, Hela.
Conjugation
unconjugated
Clone Number
SC53-07
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland. Alteration of TPH function may be a key factor in the pathology of several neuropsychiatric disorders associated with serotonin, including depression, aggression, alcoholism and schizophrenia. For instance, L-DOPA, which is used as a common therapy for Parkinson’s disease (PD) patients, inhibits TPH function, which subsequently, is thought to contribute to the onset of depression in PD patients.
Background References
1. Cheng HH et al. Quiescent and proliferative fibroblasts exhibit differential p300 HAT activation through control of 5-methoxytryptophan production. PLoS One 9:e88507 (2014).
2. Dempsie Y et al. Dexfenfluramine and the oestrogen-metabolizing enzyme CYP1B1 in the development of pulmonary arterial hypertension. Cardiovasc Res 99:24-34 (2013).
Sequence Similarity
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Tissue Specificity
Isoform 2 seems to be less widely expressed than isoform 1.
Subcellular Location
Cytosol, neuron projection.
Synonyms
Indoleacetic acid 5 hydroxylase antibody
L tryptophan hydroxylase antibody
MGC119994 antibody
TPH 1 antibody
TPH antibody
TPH1 antibody
TPH1_HUMAN antibody
TPRH antibody
TRPH antibody
Tryptophan 5 hydroxylase 1 antibody
ExpandImages
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This data was developed using ET1610-37, the same antibody clone in a different buffer formulation.
Western blot analysis of Tryptophan Hydroxylase 1 (TPH1) on different lysates with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (ET1610-37) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: LNCaP cell lysate
Lane 3: HL-60 cell lysate
Lane 4: THP-1 cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using ET1610-37, the same antibody clone in a different buffer formulation.
Western blot analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody (ET1610-37) at 1/1,000 dilution.
Lane 1: MDA-MB-231-si NT cell lysate (10 µg/Lane)
Lane 2: MDA-MB-231-si TPH1 cell lysate (10 µg/Lane)
Predicted band size: 51 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
ET1610-37 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-37, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1610-37, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (ET1610-37) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-37) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1610-37, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (ET1610-37) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-37) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1610-37, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Tryptophan Hydroxylase 1 (TPH1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1610-37, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Tryptophan Hydroxylase 1 (TPH1) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-37, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"