Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody [SC68-07] - BSA and Azide free
Catalog# HA750229
Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody [SC68-07] - BSA and Azide free
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WB
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IHC-P
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IP
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IF-Tissue
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Human
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Mouse
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unconjugated
Overview
Product Name
Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody [SC68-07] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Mast Cell Tryptase aa 10-275 / 275.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IP, IF-Tissue
Target Molecular Weight
Predicted band size: 30 kDa
Positive Control
Human lung tissue lysates, mouse skin tissue lysates, human lung tissue, human tonsil tissue, human small intestine tissue, human prostate carcinoma tissue.
Conjugation
unconjugated
Clone Number
SC68-07
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:5,000-1:10,000
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IP
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Use at an assay dependent concentration.
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IF-Tissue
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1:1,000
Target
Function
Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast cell chymase, known as CMA1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19-amino acid signal peptide, an acidic 2-amino acid propeptide and a 226-amino acid catalytic domain. Tryptases comprise a family of trypsin-like serine proteases that are enzymatically active as heparin-stabilized tetramers. There are four functional genes for tryptase: α I, β I, β II and γ I, which map to human chromosome 16p13.3, with β tryptases representing the main isoenzymes expressed in mast cells. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function.
Background References
1. De Martin S et al. Expression and distribution of the adrenomedullin system in newborn human thymus. PLoS One 9:e97592 (2014).
2. Edmunds MC et al. Paradoxical effects of heme arginate on survival of myocutaneous flaps. Am J Physiol Regul Integr Comp Physiol 306:R10-22 (2014).
Sequence Similarity
Belongs to the peptidase S1 family. Tryptase subfamily.
Tissue Specificity
Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level).
Subcellular Location
Secreted.
Synonyms
alpha II antibody
Lung tryptase antibody
Mast cell alpha II tryptase antibody
Mast cell beta I tryptase antibody
Mast cell protease 7 antibody
Mast cell protease II antibody
MCP 7 antibody
Pituitary tryptase antibody
Skin tryptase antibody
TPS 1 antibody
ExpandImages
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Western blot analysis of Mast Cell Tryptase on different lysates with Rabbit anti-Mast Cell Tryptase antibody (HA750229) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate
Lane 2: Mouse skin tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 35 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750229) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Mast Cell Tryptase antibody (HA750229) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750229) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA750229, 1/1,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"