NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
Catalog# HA750239
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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Zebrafish
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unconjugated
Overview
Product Name
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human NSE aa 30-70.
Species Reactivity
Human, Mouse, Rat (Predicted: Zebrafish)
Predicted species support after-sales service
Validated Applications
WB
Target Molecular Weight
Predicted band size: 47 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, PC-12 cell lysate, SH-SY5Y cell lysate, human breast cancer tissue , SH-SY5Y, 293, rat brain tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
SC06-28
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IF-Cell | IP | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Background References
1. Tang J et al. ENO2 drives tumor cell-induced M2 macrophage polarization to promote colorectal cancer liver metastasis. Signal Transduct Target Ther. 2026 May
2. Zhou Y et al. ENO2, a Glycolytic Enzyme, Contributes to Prostate Cancer Metastasis: A Systematic Review of Literature. Cancers (Basel). 2024 Jul
Sequence Similarity
Belongs to the enolase family.
Tissue Specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Subcellular Location
Cytoplasm, Cell membrane.
Synonyms
2 phospho D glycerate hydrolyase antibody
2-phospho-D-glycerate hydro-lyase antibody
Eno 2 antibody
ENO2 antibody
ENOG antibody
ENOG_HUMAN antibody
Enolase 2 (gamma, neuronal) antibody
Enolase 2 antibody
Enolase 2 gamma neuronal antibody
Enolase2 antibody
ExpandImages
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This data was developed using ET1610-96, the same antibody clone in a different buffer formulation.
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (ET1610-96) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: HEK-293 cell lysate (10 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: SH-SY5Y cell lysate (10 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using ET1610-96, the same antibody clone in a different buffer formulation.
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (ET1610-96) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-NSE KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-96) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Application: IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: iFluor™ 488
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Application: WB
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
