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NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750239

NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Zebrafish

Conjugation

  • unconjugated

Overview

Product Name

NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human NSE aa 30-70.

Species Reactivity

Human, Mouse, Rat (Predicted: Zebrafish)

Predicted species support after-sales service

Validated Applications

WB

Target Molecular Weight

Predicted band size: 47 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, PC-12 cell lysate, SH-SY5Y cell lysate, human breast cancer tissue , SH-SY5Y, 293, rat brain tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

SC06-28

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

Target

Function

Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Background References

1. Tang J et al. ENO2 drives tumor cell-induced M2 macrophage polarization to promote colorectal cancer liver metastasis. Signal Transduct Target Ther. 2026 May

2. Zhou Y et al. ENO2, a Glycolytic Enzyme, Contributes to Prostate Cancer Metastasis: A Systematic Review of Literature. Cancers (Basel). 2024 Jul

Sequence Similarity

Belongs to the enolase family.

Tissue Specificity

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Subcellular Location

Cytoplasm, Cell membrane.

UNIPROT

SwissProt: P09104 Human

SwissProt: P17183 Mouse

SwissProt: P07323 Rat

Synonyms

2 phospho D glycerate hydrolyase antibody

2-phospho-D-glycerate hydro-lyase antibody

Eno 2 antibody

ENO2 antibody

ENOG antibody

ENOG_HUMAN antibody

Enolase 2 (gamma, neuronal) antibody

Enolase 2 antibody

Enolase 2 gamma neuronal antibody

Enolase2 antibody

Expand

Images

  • Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (<a href="/products/HA750239" style="font-weight: bold;text-decoration: underline;">HA750239</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (10 µg/Lane)<br />Lane 2: HeLa cell lysate (10 µg/Lane)<br />Lane 3: HEK-293 cell lysate (10 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 5: PC-12 cell lysate (10 µg/Lane)<br />Lane 6: SH-SY5Y cell lysate (10 µg/Lane)<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 1 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750239" style="font-weight: bold;text-decoration: underline;">HA750239</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (HA750239) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (10 µg/Lane)
    Lane 2: HeLa cell lysate (10 µg/Lane)
    Lane 3: HEK-293 cell lysate (10 µg/Lane)
    Lane 4: Mouse brain tissue lysate (20 µg/Lane)
    Lane 5: PC-12 cell lysate (10 µg/Lane)
    Lane 6: SH-SY5Y cell lysate (10 µg/Lane)

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

    Exposure time: 1 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750239) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (<a href="/products/HA750239" style="font-weight: bold;text-decoration: underline;">HA750239</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-NSE KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750239" style="font-weight: bold;text-decoration: underline;">HA750239</a>) at 1/5,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (HA750239) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-NSE KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750239) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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