NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
Catalog# HA750239
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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ET1610-96
含抗保成分
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Zebrafish
Predicted species support after-sales service
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unconjugated
Overview
Product Name
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human NSE aa 30-70.
Species Reactivity
Human, Mouse, Rat (Predicted: Zebrafish)
Predicted species support after-sales service
Validated Applications
WB
Molecular Weight
Predicted band size: 47 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, PC-12 cell lysate, SH-SY5Y cell lysate, hybrid fish (crucian-carp) brain tissue lysate, human breast cancer tissue , SH-SY5Y, 293, rat brain tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
SC06-28
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
Target
Function
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Background References
1. Chang BS et al. Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice. J Reprod Dev 61:145-53 (2015).
2. Sun Y et al. The expression and significance of neuronal iconic proteins in podocytes. PLoS One 9:e93999 (2014).
Sequence Similarity
Belongs to the enolase family.
Tissue Specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Subcellular Location
Cytoplasm, Cell membrane.
Synonyms
2 phospho D glycerate hydrolyase antibody
2-phospho-D-glycerate hydro-lyase antibody
Eno 2 antibody
ENO2 antibody
ENOG antibody
ENOG_HUMAN antibody
Enolase 2 (gamma, neuronal) antibody
Enolase 2 antibody
Enolase 2 gamma neuronal antibody
Enolase2 antibody
ExpandImages
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Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (HA750239) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: HEK-293 cell lysate (10 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: SH-SY5Y cell lysate (10 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750239) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (HA750239) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-NSE KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750239) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of NSE on hybrid fish
(crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750239, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Application: IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: iFluor™ 488
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Application: WB
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
