Myosin Light Chain 2 Recombinant Rabbit Monoclonal Antibody [SN67-09] - BSA and Azide free
Catalog# HA750244
Myosin Light Chain 2 Recombinant Rabbit Monoclonal Antibody [SN67-09] - BSA and Azide free
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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ET1611-13
含抗保成分
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Zebrafish
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Myosin Light Chain 2 Recombinant Rabbit Monoclonal Antibody [SN67-09] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Myosin Light Chain 2 aa 117-166 / 166.
Species Reactivity
Human, Mouse, Rat (Predicted: Zebrafish)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 19 kDa
Positive Control
Mouse heart tissue lysate, Rat heart tissue lysate, hybrid fish (crucian-carp) skin tissue lysates, human striated muscle tissue, human fetal skeletal muscle tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse heart tissue, rat heart tissue.
Conjugation
unconjugated
Clone Number
SN67-09
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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IHC-P
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1:800-4,000
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IP
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Use at an assay dependent concentration.
Target
Function
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2) also known as the regulatory light chain of myosin (RLC) is a protein that in humans is encoded by the MYL2 gene. This cardiac ventricular RLC isoform is distinct from that expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B) and cardiac atrial muscle (MYL7). Ventricular myosin light chain-2 (MLC-2v) refers to the ventricular cardiac muscle form of myosin light chain 2 (Myl2). MLC-2v is a 19-KDa protein composed of 166 amino acids, that belongs to the EF-hand Ca2+ binding superfamily. MLC-2v interacts with the neck/tail region of the muscle thick filament protein myosin to regulate myosin motility and function.
Background References
1. Luo XL et al. Myosin light chain 2 marks differentiating ventricular cardiomyocytes derived from human embryonic stem cells. Pflugers Arch. 2021 Jul
2. Tsuji-Tamura K et al. Pharmacological control of angiogenesis by regulating phosphorylation of myosin light chain 2. Cell Signal. 2024 Aug
Post-translational Modification
N-terminus is methylated by METTL11A/NTM1.; Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).
Subcellular Location
Cytoplasm.
Synonyms
Cardiac myosin light chain-2 antibody
Cardiac ventricular myosin light chain 2 antibody
CMH10 antibody
MLC 2v antibody
MLC-2 antibody
MLC-2v antibody
MLC2 antibody
MLRV_HUMAN antibody
MYL 2 antibody
MYL2 antibody
ExpandImages
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Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/1,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750244) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Myosin Light Chain 2 on hybrid fish (crucian-carp) skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750244, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750244) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750244) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750244) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750244) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750244) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750244) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Myosin Light Chain 2 antibody (HA750244) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750244) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Myosin Light Chain 2 was immunoprecipitated in 0.2mg mouse heart lysate with (HA750244) at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using (HA750244) at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: mouse heart lysate (input)
Lane 2: (HA750244) IP in mouse heart lysate
Lane 3: Rabbit IgG instead of (HA750244) in mouse heart lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 15 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Myosin Light Chain 2 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
