Cytokeratin 13 Recombinant Rabbit Monoclonal Antibody [SN71-09] - BSA and Azide free
Catalog# HA750261
Cytokeratin 13 Recombinant Rabbit Monoclonal Antibody [SN71-09] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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FC
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IF-Tissue
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Human
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Mouse
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ET1611-55
含抗保成分
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Zebrafish
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Cytokeratin 13 Recombinant Rabbit Monoclonal Antibody [SN71-09] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Cytokeratin 13 aa 111-160 / 458.
Species Reactivity
Human, Mouse (Predicted: Zebrafish)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P, FC, IF-Tissue
Molecular Weight
Predicted band size: 50 kDa
Positive Control
A431 cell lysate, HaCaT cell lysate, Mouse lung tissue lysate, Mouse skin tissue lysate, A431, hybrid fish (crucian-carp) brain tissue lysates, Hela, A549, human tonsil tissue, human lung squamous cell tissue, human cervical carcinoma tissue.
Conjugation
unconjugated
Clone Number
SN71-09
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:500
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IHC-P
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1:100-1:200
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FC
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1:1,000
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IF-Tissue
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1:200
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 13 belongs to the intermediate filament family and is a heterotetramer of two type I acidic and two type II basic keratins. It is generally associated with cytokeratin 4. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder which predominantly affects noncornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa.
Background References
1. Meng Q et al. Eyelid closure in embryogenesis is required for ocular adnexa development. Invest Ophthalmol Vis Sci 55:7652-61 (2014).
2. Fulzele A et al. Proteomic profile of keratins in cancer of the gingivo buccal complex: Consolidating insights for clinical applications. J Proteomics 91C:242-258 (2013).
Sequence Similarity
Belongs to the intermediate filament family.
Tissue Specificity
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
Post-translational Modification
O-glycosylated; glycans consist of single N-acetylglucosamine residues.
Subcellular Location
Extracellular exosome, intermediate filament cytoskeleton, keratin filament, nucleus.
Synonyms
47 kDa cytokeratin antibody
CK-13 antibody
CK13 antibody
Cytokeratin 13 antibody
Cytokeratin-13 antibody
K13 antibody
K1C13_HUMAN antibody
Ka13 antibody
Keratin 13 antibody
Keratin antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Cytokeratin 13 on different lysates with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/2,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HaCaT cell lysate
Lane 3: HEK-293 (-&low) cell lysate
Lane 4: Mouse lung tissue lysate
Lane 5: Mouse skin tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750261) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of A431 (positive) and HEK-293 (negative) labeling Cytokeratin 13 with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Western blot analysis of Cytokeratin 13 on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750261, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Immunocytochemistry analysis of Hela cells labeling Cytokeratin 13 with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of A549 cells labeling Cytokeratin 13 with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750261) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/200 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750261) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung squamous cell tissue with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750261) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750261) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-Cytokeratin 13 antibody (HA750261) at 1/100 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750261) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Flow cytometric analysis of HEK-293 (left, negative) and A431 (right, positive) cells labeling Cytokeratin 13.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750261, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Application: IF-Tissue
Species: Human
Site: cervical carcinoma
Sample: Paraffin-embedded section
Antibody concentration: 1/200
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"